Directed Enzyme Evolution and High-Throughput Screening

Michael J. McLachlan, Ryan P. Sullivan, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

Original languageEnglish (US)
Title of host publicationBiocatalysis for the Pharmaceutical Industry
Subtitle of host publicationDiscovery, Development, and Manufacturing
PublisherJohn Wiley & Sons, Ltd.
Pages45-64
Number of pages20
ISBN (Print)9780470823149
DOIs
StatePublished - Sep 10 2009

Keywords

  • Directed enzyme evolution and high-throughput screening
  • Directed evolution and directed evolution practices in industry
  • Directed evolution library creation strategies
  • Directed evolution library screening/selection methods
  • DNA shuffling - for in vitro recombinant mutagenesis and diversity generation
  • Environmental impact of traditional chemical syntheses and biocatalysis
  • Enzymatic process integration into industrial settings
  • EpPCR - random mutagenesis technique
  • Error-prone polymerase chain reaction (epPCR)
  • Polymerase chain reaction (PCR)

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

McLachlan, M. J., Sullivan, R. P., & Zhao, H. (2009). Directed Enzyme Evolution and High-Throughput Screening. In Biocatalysis for the Pharmaceutical Industry: Discovery, Development, and Manufacturing (pp. 45-64). John Wiley & Sons, Ltd.. https://doi.org/10.1002/9780470823163.ch3