Direct-write assembly of 3D hydrogel scaffolds for guided cell growth

Robert A. Barry, Robert F. Shepherd, Jennifer N. Hanson, Ralph G. Nuzzo, Pierre Wiltzius, Jennifer A Lewis

Research output: Contribution to journalArticle


A new polymeric ink composed of physically entangled poly (acrylamide) chains in a photopolymerizable acrylamide solution that can be directly patterned in air through a combination of direct-write assembly and in situ photocuring was designed. Direct ink writing (DIW) is a layer-by-layer assembly technique where materials are patterned in both planar and 3D forms with lateral feature sizes achieved by ink-jet printing. The ink to be utilized is formed by mixing 1mL deionized water, 5g of glycerol, and 3.5g acrylamide, and the solution underwent spontaneous polymerization within hours to day. This novel ink design can be readily extended to other chemistries including those with more suitable for tissue engineering scaffolds. The HNMR measurements were carried out using a Varian Unity 400, with the ability to create hydrogel scaffolds with microscale features in both planar and 3D forms that opens avenue for tailoring scaffolds for wide applications in tissue engineering.

Original languageEnglish (US)
Pages (from-to)2407-2410
Number of pages4
JournalAdvanced Materials
Issue number23
StatePublished - Jun 19 2009

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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    Barry, R. A., Shepherd, R. F., Hanson, J. N., Nuzzo, R. G., Wiltzius, P., & Lewis, J. A. (2009). Direct-write assembly of 3D hydrogel scaffolds for guided cell growth. Advanced Materials, 21(23), 2407-2410.