Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

Karson S. Putt; Gregory J. Beilman; Paul J. Hergenrother

doi:10.1002/cbic.200400330

Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

Karson S. Putt, Gregory J. Beilman, Paul J. Hergenrother

Research output: Contribution to journal › Article › peer-review

Abstract

PARP-angel of death. The PARP-1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP-1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.

Original language	English (US)
Pages (from-to)	53-55
Number of pages	3
Journal	ChemBioChem
Volume	6
Issue number	1
DOIs	https://doi.org/10.1002/cbic.200400330
State	Published - Jan 2005

Keywords

Apoptosis
Bioorganic chemistry
Enzymes
Necrosis
PARP-1

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200400330

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KW - Bioorganic chemistry

KW - Enzymes

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Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

Abstract

Keywords

ASJC Scopus subject areas

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