TY - GEN
T1 - Direct measurements of adhesive interactions between cadherin extracellular domains
AU - Leckband, D.
AU - Sivasankar, S.
AU - Brieher, W.
AU - Lavrik, N.
AU - Gumbiner, B.
PY - 1999
Y1 - 1999
N2 - In this work, we examine the intermolecular alignments that mediate the homophilic adhesion between cadherin ectodomains from Xenopus. We used the surface force apparatus to measure directly the force between oriented, cadherin monolayers as a function of their separation distance. These measurements identified relative domain alignments that give rise to homophilic, adhesive interactions between proteins. They show that cadherin extracellular domains bind in at least two antiparallel configurations. The two adhesive contacts are separated by a distance equal to one cadherin domain. These results suggest that cadherin adhesion is not determined by a single binding site, but may involve several domains.
AB - In this work, we examine the intermolecular alignments that mediate the homophilic adhesion between cadherin ectodomains from Xenopus. We used the surface force apparatus to measure directly the force between oriented, cadherin monolayers as a function of their separation distance. These measurements identified relative domain alignments that give rise to homophilic, adhesive interactions between proteins. They show that cadherin extracellular domains bind in at least two antiparallel configurations. The two adhesive contacts are separated by a distance equal to one cadherin domain. These results suggest that cadherin adhesion is not determined by a single binding site, but may involve several domains.
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M3 - Conference contribution
AN - SCOPUS:0033342351
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -