Direct measurements of adhesive interactions between cadherin extracellular domains

D. Leckband, S. Sivasankar, W. Brieher, N. Lavrik, B. Gumbiner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work, we examine the intermolecular alignments that mediate the homophilic adhesion between cadherin ectodomains from Xenopus. We used the surface force apparatus to measure directly the force between oriented, cadherin monolayers as a function of their separation distance. These measurements identified relative domain alignments that give rise to homophilic, adhesive interactions between proteins. They show that cadherin extracellular domains bind in at least two antiparallel configurations. The two adhesive contacts are separated by a distance equal to one cadherin domain. These results suggest that cadherin adhesion is not determined by a single binding site, but may involve several domains.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume2
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Cadherins
Adhesives
Adhesion
Binding sites
Monolayers
Proteins
Xenopus
Binding Sites

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

Leckband, D., Sivasankar, S., Brieher, W., Lavrik, N., & Gumbiner, B. (1999). Direct measurements of adhesive interactions between cadherin extracellular domains. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 2). IEEE.

Direct measurements of adhesive interactions between cadherin extracellular domains. / Leckband, D.; Sivasankar, S.; Brieher, W.; Lavrik, N.; Gumbiner, B.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Leckband, D, Sivasankar, S, Brieher, W, Lavrik, N & Gumbiner, B 1999, Direct measurements of adhesive interactions between cadherin extracellular domains. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 2, IEEE, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Leckband D, Sivasankar S, Brieher W, Lavrik N, Gumbiner B. Direct measurements of adhesive interactions between cadherin extracellular domains. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE. 1999. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).
Leckband, D. ; Sivasankar, S. ; Brieher, W. ; Lavrik, N. ; Gumbiner, B. / Direct measurements of adhesive interactions between cadherin extracellular domains. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).
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