Direct measurement of interactions between tethered poly(ethylene glycol) chains and adsorbed mucin layers

N. V. Efremova, Y. Huang, N. A. Peppas, D. E. Leckband

Research output: Contribution to journalArticle

Abstract

Direct force measurements were used to investigate the interactions between grafted poly(ethylene glycol) (PEG) (Mw 2000) and both adsorbed and soluble bovine submaxillary gland mucin. These measurements show that both soluble and adsorbed mucin adhere weakly to the grafted PEG layers. Soluble mucin mediates a concentration-dependent, bridging attraction between the PEG films. Similarly, PEG adheres to adsorbed mucin layers in both the presence and absence of 0.2 mg/mL mucin solutions. The heteropolymer attraction is pH dependent, with the adhesion being substantially higher at pH 2 than at pH 7.2. The latter suggests that hydrogen-bonding interactions are responsible for binding. The weaker mucin interactions at pH 7.2, however, allow for PEG-coated surfaces to adhere to surface-bound mucin in the presence of soluble mucin. Such characteristics are particularly desirable for coatings for oral drug carriers.

Original languageEnglish (US)
Pages (from-to)836-845
Number of pages10
JournalLangmuir
Volume18
Issue number3
DOIs
StatePublished - Feb 5 2002

Fingerprint

Mucins
Polyethylene glycols
glycols
ethylene
attraction
interactions
glands
drugs
adhesion
Force measurement
coatings
Drug Carriers
Hydrogen bonds
hydrogen
Adhesion
Coatings

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Direct measurement of interactions between tethered poly(ethylene glycol) chains and adsorbed mucin layers. / Efremova, N. V.; Huang, Y.; Peppas, N. A.; Leckband, D. E.

In: Langmuir, Vol. 18, No. 3, 05.02.2002, p. 836-845.

Research output: Contribution to journalArticle

Efremova, N. V. ; Huang, Y. ; Peppas, N. A. ; Leckband, D. E. / Direct measurement of interactions between tethered poly(ethylene glycol) chains and adsorbed mucin layers. In: Langmuir. 2002 ; Vol. 18, No. 3. pp. 836-845.
@article{f390d9db4ce14eb1aa8ebe3da39e822b,
title = "Direct measurement of interactions between tethered poly(ethylene glycol) chains and adsorbed mucin layers",
abstract = "Direct force measurements were used to investigate the interactions between grafted poly(ethylene glycol) (PEG) (Mw 2000) and both adsorbed and soluble bovine submaxillary gland mucin. These measurements show that both soluble and adsorbed mucin adhere weakly to the grafted PEG layers. Soluble mucin mediates a concentration-dependent, bridging attraction between the PEG films. Similarly, PEG adheres to adsorbed mucin layers in both the presence and absence of 0.2 mg/mL mucin solutions. The heteropolymer attraction is pH dependent, with the adhesion being substantially higher at pH 2 than at pH 7.2. The latter suggests that hydrogen-bonding interactions are responsible for binding. The weaker mucin interactions at pH 7.2, however, allow for PEG-coated surfaces to adhere to surface-bound mucin in the presence of soluble mucin. Such characteristics are particularly desirable for coatings for oral drug carriers.",
author = "Efremova, {N. V.} and Y. Huang and Peppas, {N. A.} and Leckband, {D. E.}",
year = "2002",
month = "2",
day = "5",
doi = "10.1021/la011303p",
language = "English (US)",
volume = "18",
pages = "836--845",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Direct measurement of interactions between tethered poly(ethylene glycol) chains and adsorbed mucin layers

AU - Efremova, N. V.

AU - Huang, Y.

AU - Peppas, N. A.

AU - Leckband, D. E.

PY - 2002/2/5

Y1 - 2002/2/5

N2 - Direct force measurements were used to investigate the interactions between grafted poly(ethylene glycol) (PEG) (Mw 2000) and both adsorbed and soluble bovine submaxillary gland mucin. These measurements show that both soluble and adsorbed mucin adhere weakly to the grafted PEG layers. Soluble mucin mediates a concentration-dependent, bridging attraction between the PEG films. Similarly, PEG adheres to adsorbed mucin layers in both the presence and absence of 0.2 mg/mL mucin solutions. The heteropolymer attraction is pH dependent, with the adhesion being substantially higher at pH 2 than at pH 7.2. The latter suggests that hydrogen-bonding interactions are responsible for binding. The weaker mucin interactions at pH 7.2, however, allow for PEG-coated surfaces to adhere to surface-bound mucin in the presence of soluble mucin. Such characteristics are particularly desirable for coatings for oral drug carriers.

AB - Direct force measurements were used to investigate the interactions between grafted poly(ethylene glycol) (PEG) (Mw 2000) and both adsorbed and soluble bovine submaxillary gland mucin. These measurements show that both soluble and adsorbed mucin adhere weakly to the grafted PEG layers. Soluble mucin mediates a concentration-dependent, bridging attraction between the PEG films. Similarly, PEG adheres to adsorbed mucin layers in both the presence and absence of 0.2 mg/mL mucin solutions. The heteropolymer attraction is pH dependent, with the adhesion being substantially higher at pH 2 than at pH 7.2. The latter suggests that hydrogen-bonding interactions are responsible for binding. The weaker mucin interactions at pH 7.2, however, allow for PEG-coated surfaces to adhere to surface-bound mucin in the presence of soluble mucin. Such characteristics are particularly desirable for coatings for oral drug carriers.

UR - http://www.scopus.com/inward/record.url?scp=0037022477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037022477&partnerID=8YFLogxK

U2 - 10.1021/la011303p

DO - 10.1021/la011303p

M3 - Article

AN - SCOPUS:0037022477

VL - 18

SP - 836

EP - 845

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 3

ER -