Direct infrared detection of the covalently ring linked his-tyr structure in the active site of the heme-copper oxidases

Farol Tomson, James A. Bailey, Robert B. Gennis, Clifford J. Unkefer, Zizhong Li, Louis A. Silks, Rodolfo A. Martinez, Robert J. Donohoe, R. Brian Dyer, William H. Woodruff

Research output: Contribution to journalArticlepeer-review

Abstract

Infrared spectroscopy, isotopic labeling ([15Nδε]histidine and ring-deuterated tyrosine), synthetic model studies, and normal mode calculations are employed to search for the spectroscopic signatures of the unique, covalently linked (His Nε-Cε Tyr) biting structure in the heme-copper oxidases. The specific enzyme examined is the cytochrome b03 quinol oxidase of E. coli. Infrared features of histidine and tyrosine are identified in the frequency regions of imidazole and phenol ring stretching modes (1350-1650 cm-1) and C-H and N-H stretching modes as well. as overtones and combinations (> 3000 cm-1). Two of these, at ca. 1480 and 1550 cm-1, and their combination tones between 3010 and 3040 cm-1, are definitively identified with the biting structure involving H284 and Y288 in the E. coli enzyme. Studies of a synthetic analogue of the H-Y structure, 4-methylimidazole covalently linked to p-cresol, show that a feature near 1540 cm-1 is unique to the biring structure and is absent from the infrared spectrum of 4-methylimidazole or p-cresol alone. This feature is readily detectable by infrared difference techniques, and offers a direct spectroscopic probe for potential radical production involving the H-Y structure in the O2 reduction cycle of the oxidases.

Original languageEnglish (US)
Pages (from-to)14383-14390
Number of pages8
JournalBiochemistry
Volume41
Issue number48
DOIs
StatePublished - Dec 3 2002

ASJC Scopus subject areas

  • Biochemistry

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