Direct fermentation of Jerusalem artichoke tuber powder for production of L-lactic acid and D-lactic acid by metabolically engineered Kluyveromyces marxianus

Jung Hoon Bae, Hyun Jin Kim, Mi Jin Kim, Bong Hyun Sung, Jae Heung Jeon, Hyun Soon Kim, Yong Su Jin, Dae Hyuk Kweon, Jung Hoon Sohn

Research output: Contribution to journalArticlepeer-review

Abstract

An efficient production system for optically pure L- and D-lactic acid (LA) from Jerusalem artichoke tuber powder (JAP) was developed by metabolic engineering of Kluyveromyces marxianus. To construct LA-producing strains, the ethanol fermentation pathway of K. marxianus was redirected to LA production by disruption of KmPDC1 and expression of L- and D-lactate dehydrogenase (LDH) genes derived from Lactobacillus plantarum under the control of the K. marxianus translation elongation factor 1α promoter. To further increase the LA titer, the L-LA and D-LA consumption pathway of host strains was blocked by deletion of the oxidative LDH genes KmCYB2 and KmDLD1. The recombinant strains produced 130 g/L L-LA and 122 g/L D-LA by direct fermentation from 230 g/L JAP containing 140 g/L inulin, without pretreatment or nutrient supplementation. The conversion efficiency and optical purity were ≫>95% and ≫>99%, respectively. This system using JAP and the inulin-assimilating yeast K. marxianus could lead to a cost-effective process for the production of LA.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalJournal of Biotechnology
Volume266
DOIs
StatePublished - Jan 20 2018

Keywords

  • Inulin
  • Jerusalem artichoke
  • Kluyveromyces marxianus
  • Lactate dehydrogenase
  • Lactic acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Direct fermentation of Jerusalem artichoke tuber powder for production of L-lactic acid and D-lactic acid by metabolically engineered Kluyveromyces marxianus'. Together they form a unique fingerprint.

Cite this