TY - JOUR
T1 - Direct detection of human adenovirus or SARS-CoV-2 with ability to inform infectivity using DNA aptamer-nanopore sensors
AU - Peinetti, Ana S.
AU - Lake, Ryan J.
AU - Cong, Wen
AU - Cooper, Laura
AU - Wu, Yuting
AU - Ma, Yuan
AU - Pawel, Gregory T.
AU - Toimil-Molares, María Eugenia
AU - Trautmann, Christina
AU - Rong, Lijun
AU - Mariñas, Benito
AU - Azzaroni, Omar
AU - Lu, Yi
N1 - Funding: This work was supported by a RAPID grant from the National Science Foundation (CBET 20-29215) and a seed grant from the Institute for Sustainability, Energy, and Environment at University of Illinois at Urbana-Champaign and Illinois-JITRI Institute (JITRI 23965). A.S.P. thanks the PEW Latin American Fellowship for financial support. The irradiated PET foils are part of the experiment UMAT, which was performed at the beam line X0 at the GSI Helmholtzzentrum für Schwerionenforschung (Darmstadt, Germany) in the frame of FAIR Phase-0. O.A. acknowledges financial support from CONICET (PIP0370) and ANPCyT (PICT-2016-1680 and PICT-2017-1523).
PY - 2021/9
Y1 - 2021/9
N2 - Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 104 copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
AB - Viral infections are a major global health issue, but no current method allows rapid, direct, and ultrasensitive quantification of intact viruses with the ability to inform infectivity, causing misdiagnoses and spread of the viruses. Here, we report a method for direct detection and differentiation of infectious from noninfectious human adenovirus and SARS-CoV-2, as well as from other virus types, without any sample pretreatment. DNA aptamers are selected from a DNA library to bind intact infectious, but not noninfectious, virus and then incorporated into a solid-state nanopore, which allows strong confinement of the virus to enhance sensitivity down to 1 pfu/ml for human adenovirus and 1 × 104 copies/ml for SARS-CoV-2. Applications of the aptamer-nanopore sensors in different types of water samples, saliva, and serum are demonstrated for both enveloped and nonenveloped viruses, making the sensor generally applicable for detecting these and other emerging viruses of environmental and public health concern.
KW - COVID-19
KW - severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
UR - http://www.scopus.com/inward/record.url?scp=85115809321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85115809321&partnerID=8YFLogxK
U2 - 10.1126/sciadv.abh2848
DO - 10.1126/sciadv.abh2848
M3 - Article
C2 - 34550739
SN - 2375-2548
VL - 7
JO - Science Advances
JF - Science Advances
IS - 39
M1 - eabh2848
ER -