Abstract
The chemical complexity of cell-to-cell communication has emerged as a fundamental challenge to understanding brain systems. This is certainly true for the hypothalamus, where neuropeptide signals are heterogeneous, localized and dynamic. Thus far, most hypothalamic peptidomic studies have centered on the entire structure; however, recent advances in collection strategies and analytical technologies have enabled direct, high-resolution peptidomic profiles focused on two regions of interest, the suprachiasmatic and supraoptic nuclei, including their sub-regions and individual cells. Suites of peptides now can be identified and probed for function. High spatial and analytical sensitivities reveal that discrete hypothalamic nuclei have distinct peptidomic signatures. Peptidomic discovery not only reveals unanticipated complexity, but also peptides previously unknown that act as key circuit components. Analysis of tissue releasates identifies peptides secreted into the extracellular environment and available for transmitting intercellular signals. Direct sampling techniques define peptide-releasate profiles in spatial, temporal and event-dependent patterns. These approaches are providing remarkable new insights into the complexity of neuropeptidergic cell-to-cell signaling central to neuroendocrine physiology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 377-386 |
| Number of pages | 10 |
| Journal | Frontiers in Neuroendocrinology |
| Volume | 32 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 1 2011 |
Fingerprint
Keywords
- Arginine vasopressin (AVP)
- Gastrin-releasing peptide (GRP)
- Hypothalamus
- Little SAAS
- Mass spectrometry (MS)
- Neuropeptide
- Peptidomics
- Suprachiasmatic nucleus (SCN)
- Supraoptic nucleus (SON)
- Vasoactive intestinal peptide (VIP)
ASJC Scopus subject areas
- Endocrine and Autonomic Systems
Cite this
Direct cellular peptidomics of hypothalamic neurons. / Mitchell, Jennifer W.; Atkins, Norman; Sweedler, Jonathan V; Gillette, Martha L.
In: Frontiers in Neuroendocrinology, Vol. 32, No. 4, 01.10.2011, p. 377-386.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Direct cellular peptidomics of hypothalamic neurons
AU - Mitchell, Jennifer W.
AU - Atkins, Norman
AU - Sweedler, Jonathan V
AU - Gillette, Martha L
PY - 2011/10/1
Y1 - 2011/10/1
N2 - The chemical complexity of cell-to-cell communication has emerged as a fundamental challenge to understanding brain systems. This is certainly true for the hypothalamus, where neuropeptide signals are heterogeneous, localized and dynamic. Thus far, most hypothalamic peptidomic studies have centered on the entire structure; however, recent advances in collection strategies and analytical technologies have enabled direct, high-resolution peptidomic profiles focused on two regions of interest, the suprachiasmatic and supraoptic nuclei, including their sub-regions and individual cells. Suites of peptides now can be identified and probed for function. High spatial and analytical sensitivities reveal that discrete hypothalamic nuclei have distinct peptidomic signatures. Peptidomic discovery not only reveals unanticipated complexity, but also peptides previously unknown that act as key circuit components. Analysis of tissue releasates identifies peptides secreted into the extracellular environment and available for transmitting intercellular signals. Direct sampling techniques define peptide-releasate profiles in spatial, temporal and event-dependent patterns. These approaches are providing remarkable new insights into the complexity of neuropeptidergic cell-to-cell signaling central to neuroendocrine physiology.
AB - The chemical complexity of cell-to-cell communication has emerged as a fundamental challenge to understanding brain systems. This is certainly true for the hypothalamus, where neuropeptide signals are heterogeneous, localized and dynamic. Thus far, most hypothalamic peptidomic studies have centered on the entire structure; however, recent advances in collection strategies and analytical technologies have enabled direct, high-resolution peptidomic profiles focused on two regions of interest, the suprachiasmatic and supraoptic nuclei, including their sub-regions and individual cells. Suites of peptides now can be identified and probed for function. High spatial and analytical sensitivities reveal that discrete hypothalamic nuclei have distinct peptidomic signatures. Peptidomic discovery not only reveals unanticipated complexity, but also peptides previously unknown that act as key circuit components. Analysis of tissue releasates identifies peptides secreted into the extracellular environment and available for transmitting intercellular signals. Direct sampling techniques define peptide-releasate profiles in spatial, temporal and event-dependent patterns. These approaches are providing remarkable new insights into the complexity of neuropeptidergic cell-to-cell signaling central to neuroendocrine physiology.
KW - Arginine vasopressin (AVP)
KW - Gastrin-releasing peptide (GRP)
KW - Hypothalamus
KW - Little SAAS
KW - Mass spectrometry (MS)
KW - Neuropeptide
KW - Peptidomics
KW - Suprachiasmatic nucleus (SCN)
KW - Supraoptic nucleus (SON)
KW - Vasoactive intestinal peptide (VIP)
UR - http://www.scopus.com/inward/record.url?scp=80053127564&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053127564&partnerID=8YFLogxK
U2 - 10.1016/j.yfrne.2011.02.005
DO - 10.1016/j.yfrne.2011.02.005
M3 - Review article
C2 - 21334363
AN - SCOPUS:80053127564
VL - 32
SP - 377
EP - 386
JO - Frontiers in Neuroendocrinology
JF - Frontiers in Neuroendocrinology
SN - 0091-3022
IS - 4
ER -