Abstract

Desorption/ionization on porous silicon (DIOS) is a form of laser desorption mass spectrometry that allows for the direct mass analysis of a variety of analytes without the addition of organic matrix. Protocols are described for the direct analysis of exocrine tissue and single neurons using DIOS-MS. The atrial gland of Aplysia californica was blotted on to porous silicon and analyzed with DIOS-MS in the range m/z 1000-4000. The ability to culture invertebrate neurons directly on porous silicon is also presented. Isolated bag cells regenerated neuronal processes in culture on porous silicon. DIOS-MS allowed the direct detection of the peptides contained in individual cultured neurons indicating that with appropriate protocols, DIOS can be used with biological samples with considerable thickness.

Original languageEnglish (US)
Pages (from-to)1317-1322
Number of pages6
JournalJournal of Mass Spectrometry
Volume36
Issue number12
DOIs
StatePublished - Dec 1 2001

Keywords

  • Aplysia californica
  • Biological tissues
  • Laser desorption/ionization
  • Neuropeptides
  • Porous silicon

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Biophysics

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