Dim-2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora

Elena Kouzminova, Eric U. Selker

Research output: Contribution to journalArticlepeer-review

Abstract

To understand better the control of DNA methylation, we cloned and characterized the dim-2 gene of Neurospora crassa, the only eukaryotic gene currently known in which mutations appear to eliminate DNA methylation. The dim-2 gene is responsible for methylation in both symmetrical and asymmetrical sites. We mapped dim.2 between wc-1 and un-10 on linkage group (LG) VIIR and identified the gene by RFLP mapping and genetic complementation. Dim-2 encodes a 1454 amino acid protein including a C-terminal domain homologous to known DNA methyltransferases (MTases) and a novel N-terminal domain. Neither a deletion that removed the first 186 amino acids of the protein nor a mutation in a putative nucleotide binding site abolished function, but a single amino acid substitution in the predicted catalytic site did. Tests for repeat-induced point mutation (RIP) indicated that dim-2 does not play a role in this process, i.e. duplicated sequences are mutated in dim-2 strains, as usual, but the mutated sequences are not methylated, unlike the situation in dim-2+ strains. We conclude that dim-2 encodes an MTase that is responsible for all DNA methylation in vegetative tissues of Neurospora.

Original languageEnglish (US)
Pages (from-to)4309-4323
Number of pages15
JournalEMBO Journal
Volume20
Issue number15
DOIs
StatePublished - Aug 1 2001

Keywords

  • DNA methyltransferase
  • Dim-2
  • Methylation mutant
  • Neurospora
  • Repeat-induced point mutation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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