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Digital and Absolute Assays for Low Abundance Molecular Biomarkers
Chia Wei Kuo,
Andrew M. Smith
Bioengineering
Technology Entrepreneur Center
Materials Science and Engineering
Biomedical and Translational Sciences
Nutritional Sciences
Carl R. Woese Institute for Genomic Biology
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Keyphrases
Molecular Biomarkers
100%
Low Abundance
100%
Digital Assay
100%
Molecular Assay
66%
Biospecimens
66%
Biomolecular
33%
Molecular Targets
33%
Nucleic Acids
33%
Microfluidics
33%
Thermodynamics
33%
Depletion Region
33%
Mass Transport
33%
Noise Level
33%
Biomolecules
33%
Design Requirements
33%
Life Science Research
33%
Signal Amplification
33%
Dynamic Range
33%
Multiplexing
33%
Laboratory Measurements
33%
Molecular Probe
33%
Tissue Homogenate
33%
Molecular Transport
33%
Enhancement Mechanism
33%
Electromagnetic Coupling
33%
Single-molecule Imaging
33%
Cost Reduction
33%
Focal Volume
33%
Multi-target
33%
Robust Detection
33%
Pullout
33%
Association Reaction
33%
Sequence Method
33%
Calibration Standard
33%
Inorganic Nanocrystals
33%
Assay Platform
33%
Attomolar
33%
Reaction Mass
33%
Sensitivity Range
33%
Target Counting
33%
Mature Technology
33%
New Formats
33%
Molecular Ensemble
33%
Readout Method
33%
Conspectus
33%
Assay Design
33%
Surface Capture
33%
Optical Label
33%
Molecular Amplification
33%
Optical Barcode
33%
Flow Counting
33%
Low-abundant Biomarkers
33%
Clinical Diagnostic Assay
33%
Sample Throughput
33%
Femtomolar Concentration
33%
Engineering
Mass Transfer
100%
Noise Level
100%
Disruptions
100%
Biomolecule
100%
Dynamic Range
100%
Multiplexing
100%
Homogenate
100%
Signal Amplification
100%
Calibration Standard
100%
Electromagnetic Coupling
100%
Depletion Zone
100%
Design Constraint
100%
Reaction Mass
100%
Flow Counting
100%
Physics
Dynamic Range
100%
Molecular Cluster
100%
Nucleic Acid
100%
Multiplexing
100%
Electromagnetic Coupling
100%
Association Reaction
100%
Biochemistry, Genetics and Molecular Biology
Surface Property
100%
Solution and Solubility
66%
Optics
66%
Nucleic Acid
33%
Facilitated Diffusion
33%
Molecular Probe
33%
Single Molecule Imaging
33%
Tissue Homogenate
33%
Electromagnetism
33%
Chemistry
Label
100%
Biological Marker
100%
Statistical Ensemble
50%
Nucleic Acid
25%
Molecular Cluster
25%
Mass Transfer
25%
Molecular Probe
25%
Association Reaction
25%
Bioassay
25%
Sequencing Method
25%