TY - JOUR
T1 - Digested protein from chia seed (Salvia hispanica L) prevents obesity and associated inflammation of adipose tissue in mice fed a high-fat diet
AU - Grancieri, Mariana
AU - Verediano, Thaisa Agrizzi
AU - Sant'Ana, Cintia Tomaz
AU - de Assis, Andressa
AU - Toledo, Renata Lopes
AU - de Mejia, Elvira Gonzalez
AU - Martino, Hercia Stampini Duarte
N1 - Funding Information:
To the “Conselho Nacional de Ciência e Tecnologia Brasileira ( CNPq )” [Grant number 200739/2017-4 ] and CAPES PrInt [Grant number 88887.511857/2020-00 ] for MG’s scholarships, to CNPq for HSDM Research Productivity’s fellowships [PQ2 – Grant number 310910/2020-0 ], and Research Support (Grant number MCTIC/CNPq No. 28/2018 – Universal – 406517/2018-5). This study was financed by “Fundação de Amparo a Pesquisa de Minas Gerais” ( FAPEMIG ) – Brazil [Grant number APQ-02183-17 ] and ACES International Joint Research Program, University of Illinois – USA [Research was supported by the USDA-NIFA-HATCH project 1014457 ].
Funding Information:
To the “Conselho Nacional de Ciência e Tecnologia Brasileira (CNPq)” [Grant number 200739/2017-4] and CAPES PrInt [Grant number 88887.511857/2020-00] for MG's scholarships, to CNPq for HSDM Research Productivity's fellowships [PQ2 – Grant number 310910/2020-0], and Research Support (Grant number MCTIC/CNPq No. 28/2018 – Universal – 406517/2018-5). This study was financed by “Fundação de Amparo a Pesquisa de Minas Gerais” (FAPEMIG) – Brazil [Grant number APQ-02183-17] and ACES International Joint Research Program, University of Illinois – USA [Research was supported by the USDA-NIFA-HATCH project 1014457]. All the experimental procedures with animals were performed in accordance with the U.K. Animals (Scientific Procedures) Act, 1986 and associated guidelines, EU Directive 2010/63/EU for animal experiments, in compliance with the ethical principles for animal experimentation. The study was approved by Ethics Committee of the Federal University of Viçosa, Viçosa, MG, Brazil (Protocol number 01/2019).
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/9
Y1 - 2022/9
N2 - Background and aim: Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of proteins and peptides with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chia seed to prevent adipogenesis and adipose tissue inflammation in mice fed a high-fat diet. Methods and results: C57Bl/6 black mice were fed a high-fat diet plus DP (400 mg/kg of body/day) for 9 weeks. DP from chia seed reduced levels of plasma total cholesterol (−17.5%), LDL (−42.8%), triacylglycerides (−12.3%), % body fat (−26.98%), and waist circumference (−5.5%) in obese mice (p < 0.05). Furthermore, treatment with DP reduced (p < 0.05) adipocytes area, foci of inflammation, levels of p-NF-κB p65, PPARγ, mRNA SREBP1 (sterol regulatory element-binding transcription), and TNF-α. DP also increased mRNA adiponectin on adipose tissue in animals DP-treated, compared with no-DP-treated animals. However, mRNA LPL and HDL levels were not changed (p > 0.05). The peptides from DP had in silico high interaction with metalloproteinase-2. Conclusions: When using this experimental model, DP from chia seed had an anti-inflammatory and anti-adipogenic effect. These results suggest the effectiveness of digested proteins from chia seed against central obesity and its associated inflammation.
AB - Background and aim: Overweight and obesity are associated with the development of several health complications, such as diabetes, cancer, and cardiovascular disease. Chia seed is a rich source of proteins and peptides with potential anti-inflammatory and antioxidant effects. This study aimed to evaluate the effects of digested proteins (DP) from chia seed to prevent adipogenesis and adipose tissue inflammation in mice fed a high-fat diet. Methods and results: C57Bl/6 black mice were fed a high-fat diet plus DP (400 mg/kg of body/day) for 9 weeks. DP from chia seed reduced levels of plasma total cholesterol (−17.5%), LDL (−42.8%), triacylglycerides (−12.3%), % body fat (−26.98%), and waist circumference (−5.5%) in obese mice (p < 0.05). Furthermore, treatment with DP reduced (p < 0.05) adipocytes area, foci of inflammation, levels of p-NF-κB p65, PPARγ, mRNA SREBP1 (sterol regulatory element-binding transcription), and TNF-α. DP also increased mRNA adiponectin on adipose tissue in animals DP-treated, compared with no-DP-treated animals. However, mRNA LPL and HDL levels were not changed (p > 0.05). The peptides from DP had in silico high interaction with metalloproteinase-2. Conclusions: When using this experimental model, DP from chia seed had an anti-inflammatory and anti-adipogenic effect. These results suggest the effectiveness of digested proteins from chia seed against central obesity and its associated inflammation.
KW - Adipogenesis
KW - Adiponectin
KW - Adipose tissue
KW - Cholesterol
KW - PPARγ
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U2 - 10.1016/j.phanu.2022.100298
DO - 10.1016/j.phanu.2022.100298
M3 - Article
AN - SCOPUS:85130832261
SN - 2213-4344
VL - 21
JO - PharmaNutrition
JF - PharmaNutrition
M1 - 100298
ER -