Diffraction phase and fluorescence microscopy

Gabriel Popescu, Yong Keun Park, Kamran Badizadegan, Ramachandra R. Dasari, Michael S. Feld

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We developed diffraction phase and fluorescence microscopy for simultaneous quantitative phase and epi-fluorescence microscopy of live cells. The sub-nanometer path-length stability is demonstrated by studying cell membrane fluctuations, while the composite phase-fluorescence imaging mode is exemplified with mitotic kidney cells.

Original languageEnglish (US)
Title of host publicationFrontiers in Optics, FiO 2006
PublisherOptical Society of America (OSA)
ISBN (Print)1557528187, 9781557528186
StatePublished - Jan 1 2006
Externally publishedYes
EventFrontiers in Optics, FiO 2006 - Rochester, NY, United States
Duration: Oct 10 2006Oct 10 2006

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701


OtherFrontiers in Optics, FiO 2006
CountryUnited States
CityRochester, NY

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Popescu, G., Park, Y. K., Badizadegan, K., Dasari, R. R., & Feld, M. S. (2006). Diffraction phase and fluorescence microscopy. In Frontiers in Optics, FiO 2006 (Optics InfoBase Conference Papers). Optical Society of America (OSA). https://doi.org/10.1364/fio.2006.ftue5