TY - JOUR
T1 - Differential peptidomics assessment of strain and age differences in mice in response to acute cocaine administration
AU - Romanova, Elena V.
AU - Rubakhin, Stanislav S.
AU - Ossyra, John R.
AU - Zombeck, Jonathan A.
AU - Nosek, Michael R.
AU - Sweedler, Jonathan V.
AU - Rhodes, Justin S.
N1 - Publisher Copyright:
© 2015 International Society for Neurochemistry.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Neurochemical differences in the hypothalamic-pituitary axis between individuals and between ages may contribute to differential susceptibility to cocaine abuse. This study measured peptide levels in the pituitary gland (Pit) and lateral hypothalamus (LH) in adolescent (age 30 days) and adult (age 65 days) mice from four standard inbred strains, FVB/NJ, DBA/2J, C57BL/6J, and BALB/cByJ, which have previously been characterized for acute locomotor responses to cocaine. Individual peptide profiles were analyzed using mass spectrometric profiling and principal component analysis. Sequences of assigned peptides were verified by tandem mass spectrometry. Principal component analysis classified all strains according to their distinct peptide profiles in Pit samples from adolescent mice, but not adults. Select pro-opiomelanocortin-derived peptides were significantly higher in adolescent BALB/cByJ and DBA/2J mice than in FVB/NJ or C57BL/6J mice. A subset of peptides in the LH, but not in the Pit, was altered by cocaine in adolescents. A 15 mg/kg dose of cocaine induced greater peptide alterations than a 30 mg/kg dose, particularly in FVB/NJ animals, with larger differences in adolescents than adults. Neuropeptides in the LH affected by acute cocaine administration included pro-opiomelanocortin-, myelin basic protein-, and glutamate transporter-derived peptides. The observed peptide differences could contribute to differential behavioral sensitivity to cocaine among strains and ages.
AB - Neurochemical differences in the hypothalamic-pituitary axis between individuals and between ages may contribute to differential susceptibility to cocaine abuse. This study measured peptide levels in the pituitary gland (Pit) and lateral hypothalamus (LH) in adolescent (age 30 days) and adult (age 65 days) mice from four standard inbred strains, FVB/NJ, DBA/2J, C57BL/6J, and BALB/cByJ, which have previously been characterized for acute locomotor responses to cocaine. Individual peptide profiles were analyzed using mass spectrometric profiling and principal component analysis. Sequences of assigned peptides were verified by tandem mass spectrometry. Principal component analysis classified all strains according to their distinct peptide profiles in Pit samples from adolescent mice, but not adults. Select pro-opiomelanocortin-derived peptides were significantly higher in adolescent BALB/cByJ and DBA/2J mice than in FVB/NJ or C57BL/6J mice. A subset of peptides in the LH, but not in the Pit, was altered by cocaine in adolescents. A 15 mg/kg dose of cocaine induced greater peptide alterations than a 30 mg/kg dose, particularly in FVB/NJ animals, with larger differences in adolescents than adults. Neuropeptides in the LH affected by acute cocaine administration included pro-opiomelanocortin-, myelin basic protein-, and glutamate transporter-derived peptides. The observed peptide differences could contribute to differential behavioral sensitivity to cocaine among strains and ages.
KW - adolescent
KW - cocaine
KW - label-free quantitation
KW - matrix-assisted laser desorption ionization MS
KW - peptidomics
KW - principal component analysis
UR - http://www.scopus.com/inward/record.url?scp=84955207012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955207012&partnerID=8YFLogxK
U2 - 10.1111/jnc.13265
DO - 10.1111/jnc.13265
M3 - Article
C2 - 26223348
AN - SCOPUS:84955207012
SN - 0022-3042
VL - 135
SP - 1038
EP - 1048
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -