Differential immunocompetence of macrophage derived using macrophage or granulocyte-macrophage colony-stimulating factor

M. S. Rutherford, L. B. Schook

Research output: Contribution to journalArticlepeer-review


Macrophages derived in vitro from bone marrow progenitors (bone marrow-derived macrophages, BMDMs) using either macrophage colony-stimulating factor (CSF-1) or granulocyte-macrophage colony-stimulating factor (GM-CSF) as the myelopoietic stimulus display differential functional, morphological, and mRNA phenotypes. The data presented here demonstrate further that CSF-1- and GM-CSF-derived BMDMs differ in immunologic capacity. GM-CSF-derived BMDMs, when compared to CSF-1-derived BMDMs, showed greater cytolytic activity against tumor necrosis factor α (TNF-α)-resistant, but not TNF-α-sensitive, tumor targets. In contrast, CSF-1-derived BMDMs produced nitrite in response to lipopolysaccharide (LPS) alone, whereas GM-CSF-derived BMDMs required interferon γ plus LPS treatment. The two BMDM populations also showed differential sensitivities to LPS for secretion of TNF-α and nitrite, but the maximal inducible amounts of these factors and prostaglandin E2 were similar between the BMDM populations. Lastly, GM-CSF-derived but not CSF-1-derived BMDMs showed an L-arginine-dependent listeriacidal activity. These results show that the functional heterogeneity of CSF-1- and GM-CSF-derived macrophages is limited and appears to result largely from differences in the activational signals required by each BMDM population to elicit a given function.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalJournal of Leukocyte Biology
Issue number1
StatePublished - 1992


  • granulocyte-macrophage colony-stimulating factor
  • macrophage colony-stimulating factor
  • macrophage immunocompetence

ASJC Scopus subject areas

  • Cell Biology


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