Differential hydrogen bonding in human CYP17 dictates hydroxylation versus lyase chemistry

Michael Gregory, Piotr J. Mak, Stephen G. Sligar, James R. Kincaid

Research output: Contribution to journalArticlepeer-review

Abstract

Consequences of alternative H-bonding: Raman spectra of oxygenated intermediates of Nanodisc-incorporated human CYP17 in the presence of natural substrates (pregnenolone and progesterone) directly confirm that substrate structure effectively alters hydrogen-bonding interactions with the critical Fe-O-O fragment and dictates its predisposition for one of two alternative reaction pathways. Such substrate control has profound physiological implications.

Original languageEnglish (US)
Pages (from-to)5342-5345
Number of pages4
JournalAngewandte Chemie - International Edition
Volume52
Issue number20
DOIs
StatePublished - May 10 2013

Keywords

  • Raman spectroscopy
  • biophysics
  • heme proteins
  • lyases
  • oxidoreductases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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