Abstract
Consequences of alternative H-bonding: Raman spectra of oxygenated intermediates of Nanodisc-incorporated human CYP17 in the presence of natural substrates (pregnenolone and progesterone) directly confirm that substrate structure effectively alters hydrogen-bonding interactions with the critical Fe-O-O fragment and dictates its predisposition for one of two alternative reaction pathways. Such substrate control has profound physiological implications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5342-5345 |
| Number of pages | 4 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 52 |
| Issue number | 20 |
| DOIs | |
| State | Published - May 10 2013 |
Keywords
- Raman spectroscopy
- biophysics
- heme proteins
- lyases
- oxidoreductases
ASJC Scopus subject areas
- Catalysis
- General Chemistry