Abstract
Consequences of alternative H-bonding: Raman spectra of oxygenated intermediates of Nanodisc-incorporated human CYP17 in the presence of natural substrates (pregnenolone and progesterone) directly confirm that substrate structure effectively alters hydrogen-bonding interactions with the critical Fe-O-O fragment and dictates its predisposition for one of two alternative reaction pathways. Such substrate control has profound physiological implications.
Original language | English (US) |
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Pages (from-to) | 5342-5345 |
Number of pages | 4 |
Journal | Angewandte Chemie - International Edition |
Volume | 52 |
Issue number | 20 |
DOIs | |
State | Published - May 10 2013 |
Keywords
- Raman spectroscopy
- biophysics
- heme proteins
- lyases
- oxidoreductases
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)