Differential expression and induction of two Drosophila cytochrome P450 genes near the Rst(2)DDTlocus

A. Brandt; M. Scharf; J. H.F. Pedra; G. Holmes; A. Dean; M. Kreitman; Barry Robert Pittendrigh

doi:10.1046/j.1365-2583.2002.00344.x

Differential expression and induction of two Drosophila cytochrome P450 genes near the Rst(2)DDTlocus

A. Brandt, M. Scharf, J. H.F. Pedra, G. Holmes, A. Dean, M. Kreitman, Barry Robert Pittendrigh

Entomology

Research output: Contribution to journal › Article › peer-review

Abstract

Previous studies have shown that the major metabolic resistance locus in the insecticide-resistant Drosophila line Rst(2)DDT^Wisconsin maps between the markers cn and vg on chromosome 2. Six cytochrome P450 genes exist in this region. We investigated the expression levels of these P450 genes in DDT-resistant and -susceptible fly lines. We report: (i) DDT resistance is significant (> 30-fold) and dominant, (ii) resistance is reduced by the cytochrome P450 inhibitor PBO, (iii) there is constitutive over-expression relative to susceptible flies of two genes encoding cytochrome P450 enzymes within the cn-vg region (CYP6G1 = 4.3-fold; CYP12D1 = 6.0-fold), and (iv) exposure to DDT results in an increased expression of only one of these two P450 genes (CYP12D1 ≥ 6-fold above constitutive resistant fly baselines).

Original language	English (US)
Pages (from-to)	337-341
Number of pages	5
Journal	Insect Molecular Biology
Volume	11
Issue number	4
DOIs	https://doi.org/10.1046/j.1365-2583.2002.00344.x
State	Published - Aug 24 2002

Keywords

CYP12D1
CYP6G1
Cytochrome
Drosophila
Induction
Insecticide
Melanogaster
P450
Pesticide
Resistance

ASJC Scopus subject areas

Molecular Biology
Genetics
Insect Science

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title = "Differential expression and induction of two Drosophila cytochrome P450 genes near the Rst(2)DDTlocus",

abstract = "Previous studies have shown that the major metabolic resistance locus in the insecticide-resistant Drosophila line Rst(2)DDTWisconsin maps between the markers cn and vg on chromosome 2. Six cytochrome P450 genes exist in this region. We investigated the expression levels of these P450 genes in DDT-resistant and -susceptible fly lines. We report: (i) DDT resistance is significant (> 30-fold) and dominant, (ii) resistance is reduced by the cytochrome P450 inhibitor PBO, (iii) there is constitutive over-expression relative to susceptible flies of two genes encoding cytochrome P450 enzymes within the cn-vg region (CYP6G1 = 4.3-fold; CYP12D1 = 6.0-fold), and (iv) exposure to DDT results in an increased expression of only one of these two P450 genes (CYP12D1 ≥ 6-fold above constitutive resistant fly baselines).",

keywords = "CYP12D1, CYP6G1, Cytochrome, Drosophila, Induction, Insecticide, Melanogaster, P450, Pesticide, Resistance",

author = "A. Brandt and M. Scharf and Pedra, {J. H.F.} and G. Holmes and A. Dean and M. Kreitman and Pittendrigh, {Barry Robert}",

year = "2002",

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AU - Brandt, A.

AU - Scharf, M.

AU - Pedra, J. H.F.

AU - Holmes, G.

AU - Dean, A.

AU - Kreitman, M.

AU - Pittendrigh, Barry Robert

PY - 2002/8/24

Y1 - 2002/8/24

N2 - Previous studies have shown that the major metabolic resistance locus in the insecticide-resistant Drosophila line Rst(2)DDTWisconsin maps between the markers cn and vg on chromosome 2. Six cytochrome P450 genes exist in this region. We investigated the expression levels of these P450 genes in DDT-resistant and -susceptible fly lines. We report: (i) DDT resistance is significant (> 30-fold) and dominant, (ii) resistance is reduced by the cytochrome P450 inhibitor PBO, (iii) there is constitutive over-expression relative to susceptible flies of two genes encoding cytochrome P450 enzymes within the cn-vg region (CYP6G1 = 4.3-fold; CYP12D1 = 6.0-fold), and (iv) exposure to DDT results in an increased expression of only one of these two P450 genes (CYP12D1 ≥ 6-fold above constitutive resistant fly baselines).

AB - Previous studies have shown that the major metabolic resistance locus in the insecticide-resistant Drosophila line Rst(2)DDTWisconsin maps between the markers cn and vg on chromosome 2. Six cytochrome P450 genes exist in this region. We investigated the expression levels of these P450 genes in DDT-resistant and -susceptible fly lines. We report: (i) DDT resistance is significant (> 30-fold) and dominant, (ii) resistance is reduced by the cytochrome P450 inhibitor PBO, (iii) there is constitutive over-expression relative to susceptible flies of two genes encoding cytochrome P450 enzymes within the cn-vg region (CYP6G1 = 4.3-fold; CYP12D1 = 6.0-fold), and (iv) exposure to DDT results in an increased expression of only one of these two P450 genes (CYP12D1 ≥ 6-fold above constitutive resistant fly baselines).

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KW - Resistance

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