Differential Adhesion Selection for Enrichment of Tendon-Derived Progenitor Cells during in Vitro Culture

Sushmitha Durgam, Brooke Schuster, Anna Cymerman, Allison Stewart, Matthew Stewart

Research output: Contribution to journalArticle

Abstract

Preplating, a technique used to separate rapidly adherent fibroblasts from the less-adherent progenitor cells, has been used successfully to isolate skeletal muscle-derived stem cells. The objective of this study was to determine if preplating could also be applied to enrich tendon-derived progenitor cells (TDPCs) before monolayer expansion. Cell suspensions obtained by collagenase digestion of equine lateral digital extensor tendon were serially transferred into adherent plates every 12 h for 4 days. TDPC fractions obtained from initial (TPP0), third (TPP3), and seventh (TPP7) preplate were passaged twice and used for subsequent analyses. Growth/proliferation and basal tenogenic gene expression of the three TDPC fractions were largely similar. Preplating and subsequent monolayer expansion did not alter the immunophenotype (CD29+, CD44+, CD90+, and CD45-) and trilineage differentiation capacity of TDPC fractions. Overall, TDPCs were robustly osteogenic, but exhibited comparatively weak adipogenic and chondrogenic capacities. These outcomes indicate that preplating does not enrich for tendon-derived progenitors during in vitro culture, and "whole tendon digest"-derived cells are as appropriate for cell-based therapies.

Original languageEnglish (US)
Pages (from-to)801-808
Number of pages8
JournalTissue Engineering - Part C: Methods
Volume22
Issue number8
DOIs
StatePublished - Aug 1 2016

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

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