Different strategies adopted by Kb and Ld to generate T cell specificity directed against their respective bound peptides

Natalie A. Bowerman, Leremy A. Colf, K. Cristopher Garcia, David M Kranz

Research output: Contribution to journalArticle

Abstract

Mouse T cell clone 2C recognizes two different major histocompatibility (MHC) ligands, the self MHC Kb and the allogeneic MHC Ld. Two distinct peptides, SIY (SIYRYYGL) and QL9 (QLSPFPFDL), act as strong and specific agonists when bound to Kb and Ld, respectively. To explore further the mechanisms involved in peptide potency and specificity, here we examined a collection of single amino acid peptide variants of SIY and QL9 for 1) T cell activity, 2) binding to their respective MHC, and 3) binding to the 2C T cell receptor (TCR) and high affinity TCR mutants. Characterization of SIY binding to MHC Kb revealed significant effects of three SIY residues that were clearly embedded within the Kb molecule. In contrast, QL9 binding to MHCLd was influenced by the majority of peptide side chains, distributed across the entire length of the peptide. Binding of the SIY-Kb complex to the TCR involved three SIY residues that were pointed toward the TCR, whereas again the majority of QL9 residues influenced binding of TCRs, and thus the QL9 residues had impacts on both Ld and TCR binding. In general, the magnitude of T cell activity mediated by a peptide variant was influenced more by peptide binding to MHC than by binding the TCR, especially for higher affinity TCRs. Findings with both systems, but QL9-Ld in particular, suggest that many single-residue substitutions, introduced into peptides to improve their binding to MHC and thus their vaccine potential, could impair T cell reactivity due to their dual impact on TCR binding.

Original languageEnglish (US)
Pages (from-to)32551-32561
Number of pages11
JournalJournal of Biological Chemistry
Volume284
Issue number47
DOIs
StatePublished - Nov 20 2009

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T-Cell Antigen Receptor Specificity
T-cells
Histocompatibility
T-Cell Antigen Receptor
Peptides
T-Lymphocytes
Substitution reactions
Vaccines
Clone Cells
Ligands
Amino Acids
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Different strategies adopted by Kb and Ld to generate T cell specificity directed against their respective bound peptides. / Bowerman, Natalie A.; Colf, Leremy A.; Garcia, K. Cristopher; Kranz, David M.

In: Journal of Biological Chemistry, Vol. 284, No. 47, 20.11.2009, p. 32551-32561.

Research output: Contribution to journalArticle

Bowerman, Natalie A. ; Colf, Leremy A. ; Garcia, K. Cristopher ; Kranz, David M. / Different strategies adopted by Kb and Ld to generate T cell specificity directed against their respective bound peptides. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 47. pp. 32551-32561.
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