TY - JOUR
T1 - Differences in immune cell function between tuberculosis positive and negative Asian elephants
AU - Landolfi, Jennifer A.
AU - Miller, Michele
AU - Maddox, Carol
AU - Zuckermann, Federico
AU - Langan, Jennifer N.
AU - Terio, Karen A.
N1 - Funding Information:
The authors would like to thank the institutions and associated staff that contributed to this work through the donation of samples. The authors would also like to specifically acknowledge Ringling Brothers Center for Elephant Conservation and the Performing Animal Welfare Society. This study was supported by Morris Animal Foundation grant D09ZO-025 and a grant provided by the Chicago Zoological Society/Chicago Board of Trade Endangered Species Fund .
Funding Information:
As stated in the above Acknowledgments section, funding support was provided by Morris Animal Foundation Grant D09ZO-025 and a grant from the Chicago Zoological Society/Chicago Board of Trade Endangered Species Fund.
PY - 2014/7
Y1 - 2014/7
N2 - Tuberculosis is an important health concern for Asian elephant (Elephas maximus) populations worldwide, however, mechanisms underlying susceptibility to Mycobacterium tuberculosis are unknown. Proliferative responses assessed via brominated uridine incorporation and cytokine expression measured by real-time RT-PCR were evaluated in peripheral blood mononuclear cell (PBMC) cultures from 8 tuberculosis negative and 8 positive Asian elephants. Cultures were stimulated with Mycobacterium bovis purified protein derivative (PPD-B), M. tuberculosis culture filtrate protein (CFP)-10, and Mycobacterium avium PPD (PPD-A). Following stimulation with PPD-B, proliferation was higher (α = 0.005) in positive samples; no significant differences were detected following CFP-10 or PPD-A stimulation. Tumor necrosis factor (TNF)-α, interleukin (IL)-12, and interferon (IFN)-γ expression was greater in samples from positive elephants following stimulation with PPD-B (α = 0.025) and CFP-10 (α = 0.025 TNF-α and IL-12; α = 0.005 IFN-γ). Stimulation with PPD-A also produced enhanced IL-12 expression in positive samples (α = 0.025). Findings suggested that differences in immune cell function exist between tuberculosis positive and negative elephants. Proliferative responses and expression of TNF-α, IL-12, and IFN-γ in response to stimulation with PPD-B and CFP-10 differ between tuberculosis positive and negative elephants, suggesting these parameters may be important to tuberculosis immunopathogenesis in this species.
AB - Tuberculosis is an important health concern for Asian elephant (Elephas maximus) populations worldwide, however, mechanisms underlying susceptibility to Mycobacterium tuberculosis are unknown. Proliferative responses assessed via brominated uridine incorporation and cytokine expression measured by real-time RT-PCR were evaluated in peripheral blood mononuclear cell (PBMC) cultures from 8 tuberculosis negative and 8 positive Asian elephants. Cultures were stimulated with Mycobacterium bovis purified protein derivative (PPD-B), M. tuberculosis culture filtrate protein (CFP)-10, and Mycobacterium avium PPD (PPD-A). Following stimulation with PPD-B, proliferation was higher (α = 0.005) in positive samples; no significant differences were detected following CFP-10 or PPD-A stimulation. Tumor necrosis factor (TNF)-α, interleukin (IL)-12, and interferon (IFN)-γ expression was greater in samples from positive elephants following stimulation with PPD-B (α = 0.025) and CFP-10 (α = 0.025 TNF-α and IL-12; α = 0.005 IFN-γ). Stimulation with PPD-A also produced enhanced IL-12 expression in positive samples (α = 0.025). Findings suggested that differences in immune cell function exist between tuberculosis positive and negative elephants. Proliferative responses and expression of TNF-α, IL-12, and IFN-γ in response to stimulation with PPD-B and CFP-10 differ between tuberculosis positive and negative elephants, suggesting these parameters may be important to tuberculosis immunopathogenesis in this species.
KW - Cytokine
KW - Elephant
KW - Elephas maximus
KW - Immunity
KW - Tuberculosis
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U2 - 10.1016/j.tube.2014.03.001
DO - 10.1016/j.tube.2014.03.001
M3 - Article
C2 - 24836563
AN - SCOPUS:84902544863
SN - 1472-9792
VL - 94
SP - 374
EP - 382
JO - Bulletin of the International Union Against Tuberculosis and Lung Disease
JF - Bulletin of the International Union Against Tuberculosis and Lung Disease
IS - 4
ER -