Differences in antigen recognition and cytolytic activity of CD8+ and CD8- T cells that express the same antigen-specific receptor

Bryan K. Cho, Kuo Chiang Lian, Peter Lee, Anders Brunmark, Carol McKinley, Jianzhu Chen, David M. Kranz, Herman N. Eisen

Research output: Contribution to journalArticle

Abstract

CD8+ and CD8- T cell lines expressing the same antigen-specific receptor [the 2C T cell receptor (TCR)] were compared for ability to bind soluble peptide-MHC and to lyse target cells. The 2C TCR on CD8- cells bound a syngeneic MHC (Kb+)-peptide complex 10-100 times less well than the same TCR on CD8+ cells, and the CD8- 2C cells lysed target cells presenting this complex very poorly. Surprisingly, however, the CD8- cells differed little from CD8+ cells in ability to bind an allogeneic MHC (Ld+)-peptide complex and to lyse target cells presenting this complex. The CD8+/CD8- difference provided an opportunity to estimate how long TCR engagements with peptide-MHC have to persist to initiate the cytolytic T cell response.

Original languageEnglish (US)
Pages (from-to)1723-1727
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number4
DOIs
StatePublished - Feb 13 2001

ASJC Scopus subject areas

  • General

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