CD8+ and CD8- T cell lines expressing the same antigen-specific receptor [the 2C T cell receptor (TCR)] were compared for ability to bind soluble peptide-MHC and to lyse target cells. The 2C TCR on CD8- cells bound a syngeneic MHC (Kb+)-peptide complex 10-100 times less well than the same TCR on CD8+ cells, and the CD8- 2C cells lysed target cells presenting this complex very poorly. Surprisingly, however, the CD8- cells differed little from CD8+ cells in ability to bind an allogeneic MHC (Ld+)-peptide complex and to lyse target cells presenting this complex. The CD8+/CD8- difference provided an opportunity to estimate how long TCR engagements with peptide-MHC have to persist to initiate the cytolytic T cell response.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Feb 13 2001|
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