Abstract
CD8+ and CD8- T cell lines expressing the same antigen-specific receptor [the 2C T cell receptor (TCR)] were compared for ability to bind soluble peptide-MHC and to lyse target cells. The 2C TCR on CD8- cells bound a syngeneic MHC (Kb+)-peptide complex 10-100 times less well than the same TCR on CD8+ cells, and the CD8- 2C cells lysed target cells presenting this complex very poorly. Surprisingly, however, the CD8- cells differed little from CD8+ cells in ability to bind an allogeneic MHC (Ld+)-peptide complex and to lyse target cells presenting this complex. The CD8+/CD8- difference provided an opportunity to estimate how long TCR engagements with peptide-MHC have to persist to initiate the cytolytic T cell response.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1723-1727 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 98 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 13 2001 |
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Differences in antigen recognition and cytolytic activity of CD8+ and CD8- T cells that express the same antigen-specific receptor. / Cho, Bryan K.; Lian, Kuo Chiang; Lee, Peter; Brunmark, Anders; McKinley, Carol; Chen, Jianzhu; Kranz, David M.; Eisen, Herman N.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 4, 13.02.2001, p. 1723-1727.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differences in antigen recognition and cytolytic activity of CD8+ and CD8- T cells that express the same antigen-specific receptor
AU - Cho, Bryan K.
AU - Lian, Kuo Chiang
AU - Lee, Peter
AU - Brunmark, Anders
AU - McKinley, Carol
AU - Chen, Jianzhu
AU - Kranz, David M.
AU - Eisen, Herman N.
PY - 2001/2/13
Y1 - 2001/2/13
N2 - CD8+ and CD8- T cell lines expressing the same antigen-specific receptor [the 2C T cell receptor (TCR)] were compared for ability to bind soluble peptide-MHC and to lyse target cells. The 2C TCR on CD8- cells bound a syngeneic MHC (Kb+)-peptide complex 10-100 times less well than the same TCR on CD8+ cells, and the CD8- 2C cells lysed target cells presenting this complex very poorly. Surprisingly, however, the CD8- cells differed little from CD8+ cells in ability to bind an allogeneic MHC (Ld+)-peptide complex and to lyse target cells presenting this complex. The CD8+/CD8- difference provided an opportunity to estimate how long TCR engagements with peptide-MHC have to persist to initiate the cytolytic T cell response.
AB - CD8+ and CD8- T cell lines expressing the same antigen-specific receptor [the 2C T cell receptor (TCR)] were compared for ability to bind soluble peptide-MHC and to lyse target cells. The 2C TCR on CD8- cells bound a syngeneic MHC (Kb+)-peptide complex 10-100 times less well than the same TCR on CD8+ cells, and the CD8- 2C cells lysed target cells presenting this complex very poorly. Surprisingly, however, the CD8- cells differed little from CD8+ cells in ability to bind an allogeneic MHC (Ld+)-peptide complex and to lyse target cells presenting this complex. The CD8+/CD8- difference provided an opportunity to estimate how long TCR engagements with peptide-MHC have to persist to initiate the cytolytic T cell response.
UR - http://www.scopus.com/inward/record.url?scp=0035852736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035852736&partnerID=8YFLogxK
U2 - 10.1073/pnas.98.4.1723
DO - 10.1073/pnas.98.4.1723
M3 - Article
C2 - 11172018
AN - SCOPUS:0035852736
VL - 98
SP - 1723
EP - 1727
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 4
ER -