Dietary prebiotics, milk fat globule membrane, and lactoferrin affects structural neurodevelopment in the young piglet

Austin T. Mudd, Lindsey S. Alexander, Kirsten Berding, Rosaline V. Waworuntu, Brian M. Berg, Sharon M. Donovan, Ryan N. Dilger

Research output: Contribution to journalArticle

Abstract

Introduction: Milk fat globule membrane (MFGM) and lactoferrin have been identified as two components that have potential to affect neurodevelopment. While concentrations of some MFGM constituents in infant formulas are within human milk range, they may not be present at optimal or clinically effective levels. However, lactoferrin levels of infant formulas are consistently reported to be lower than human milk. This study sought to provide a novel combination of prebiotics, bovine-derived MFGM, and lactoferrin and assess their influence on neurodevelopment. Methods: Twenty-four male piglets were provided either TEST (n = 12) or CONT (n = 12) diet from 2 to 31 days of age. Piglets underwent spatial T-maze assessment starting at 17 days of age, were subjected to magnetic resonance imaging at 30 days of age, and were euthanized for tissue collection at 31 days of age. Results: Diffusion tensor imaging revealed differences in radial (P = 0.032) and mean (P = 0.028) diffusivities in the internal capsule, where CONT piglets had higher rates of diffusion compared with TEST piglets. Voxel-based morphometry indicated larger (P < 0.05) differences in cortical gray and white matter concentrations, with CONT piglets having larger tissue clusters in these regions compared with TEST piglets. In the spatial T-maze assessment, CONT piglets exhibited shorter latency to choice compared with TEST piglets on day 2 of acquisition and days 3 and 4 of reversal. Conclusion: Observed differences in microstructure maturation of the internal capsule and cortical tissue concentrations suggest that piglets provided TEST diet were more advanced developmentally than piglets provided CONT diet. Therefore, supplementation of infant formula with prebiotics, MFGM, and lactoferrin may support neurodevelopment in human infants.

Original languageEnglish (US)
Article number4
JournalFrontiers in Pediatrics
Volume4
Issue numberFEB
DOIs
StatePublished - Feb 1 2016

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Prebiotics
Lactoferrin
Infant Formula
Internal Capsule
Membranes
Human Milk
Diet
Diffusion Tensor Imaging
Magnetic Resonance Imaging
milk fat globule

Keywords

  • Brain
  • Internal capsule
  • Lactoferrin
  • Milk fat globule membrane
  • Neurodevelopment
  • Nutrition
  • Pig
  • Prebiotics

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Dietary prebiotics, milk fat globule membrane, and lactoferrin affects structural neurodevelopment in the young piglet. / Mudd, Austin T.; Alexander, Lindsey S.; Berding, Kirsten; Waworuntu, Rosaline V.; Berg, Brian M.; Donovan, Sharon M.; Dilger, Ryan N.

In: Frontiers in Pediatrics, Vol. 4, No. FEB, 4, 01.02.2016.

Research output: Contribution to journalArticle

Mudd, Austin T. ; Alexander, Lindsey S. ; Berding, Kirsten ; Waworuntu, Rosaline V. ; Berg, Brian M. ; Donovan, Sharon M. ; Dilger, Ryan N. / Dietary prebiotics, milk fat globule membrane, and lactoferrin affects structural neurodevelopment in the young piglet. In: Frontiers in Pediatrics. 2016 ; Vol. 4, No. FEB.
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abstract = "Introduction: Milk fat globule membrane (MFGM) and lactoferrin have been identified as two components that have potential to affect neurodevelopment. While concentrations of some MFGM constituents in infant formulas are within human milk range, they may not be present at optimal or clinically effective levels. However, lactoferrin levels of infant formulas are consistently reported to be lower than human milk. This study sought to provide a novel combination of prebiotics, bovine-derived MFGM, and lactoferrin and assess their influence on neurodevelopment. Methods: Twenty-four male piglets were provided either TEST (n = 12) or CONT (n = 12) diet from 2 to 31 days of age. Piglets underwent spatial T-maze assessment starting at 17 days of age, were subjected to magnetic resonance imaging at 30 days of age, and were euthanized for tissue collection at 31 days of age. Results: Diffusion tensor imaging revealed differences in radial (P = 0.032) and mean (P = 0.028) diffusivities in the internal capsule, where CONT piglets had higher rates of diffusion compared with TEST piglets. Voxel-based morphometry indicated larger (P < 0.05) differences in cortical gray and white matter concentrations, with CONT piglets having larger tissue clusters in these regions compared with TEST piglets. In the spatial T-maze assessment, CONT piglets exhibited shorter latency to choice compared with TEST piglets on day 2 of acquisition and days 3 and 4 of reversal. Conclusion: Observed differences in microstructure maturation of the internal capsule and cortical tissue concentrations suggest that piglets provided TEST diet were more advanced developmentally than piglets provided CONT diet. Therefore, supplementation of infant formula with prebiotics, MFGM, and lactoferrin may support neurodevelopment in human infants.",
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T1 - Dietary prebiotics, milk fat globule membrane, and lactoferrin affects structural neurodevelopment in the young piglet

AU - Mudd, Austin T.

AU - Alexander, Lindsey S.

AU - Berding, Kirsten

AU - Waworuntu, Rosaline V.

AU - Berg, Brian M.

AU - Donovan, Sharon M.

AU - Dilger, Ryan N.

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AB - Introduction: Milk fat globule membrane (MFGM) and lactoferrin have been identified as two components that have potential to affect neurodevelopment. While concentrations of some MFGM constituents in infant formulas are within human milk range, they may not be present at optimal or clinically effective levels. However, lactoferrin levels of infant formulas are consistently reported to be lower than human milk. This study sought to provide a novel combination of prebiotics, bovine-derived MFGM, and lactoferrin and assess their influence on neurodevelopment. Methods: Twenty-four male piglets were provided either TEST (n = 12) or CONT (n = 12) diet from 2 to 31 days of age. Piglets underwent spatial T-maze assessment starting at 17 days of age, were subjected to magnetic resonance imaging at 30 days of age, and were euthanized for tissue collection at 31 days of age. Results: Diffusion tensor imaging revealed differences in radial (P = 0.032) and mean (P = 0.028) diffusivities in the internal capsule, where CONT piglets had higher rates of diffusion compared with TEST piglets. Voxel-based morphometry indicated larger (P < 0.05) differences in cortical gray and white matter concentrations, with CONT piglets having larger tissue clusters in these regions compared with TEST piglets. In the spatial T-maze assessment, CONT piglets exhibited shorter latency to choice compared with TEST piglets on day 2 of acquisition and days 3 and 4 of reversal. Conclusion: Observed differences in microstructure maturation of the internal capsule and cortical tissue concentrations suggest that piglets provided TEST diet were more advanced developmentally than piglets provided CONT diet. Therefore, supplementation of infant formula with prebiotics, MFGM, and lactoferrin may support neurodevelopment in human infants.

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KW - Lactoferrin

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KW - Neurodevelopment

KW - Nutrition

KW - Pig

KW - Prebiotics

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