TY - JOUR
T1 - Dietary lycopene downregulates carotenoid 15,15′-monooxygenase and PPAR-γ in selected rat tissues
AU - Zaripheh, Susan
AU - Nara, Takayuki Y.
AU - Nakamura, Manabu T.
AU - Erdman, John W.
PY - 2006/4
Y1 - 2006/4
N2 - In vitro studies have suggested that lycopene is an efficient substrate for carotenoid 9′10′-monooxygenase II (CMO2) but an inhibitor of carotenoid 15,15′-monooxygenase I (CMO1). The objectives of this study were to clone the rat CMO2 gene, determine whether feeding lycopene for different lengths of time (3-37 d) altered the expression of genes related to carotenoid cleavage [CMO1, CMO2 and peroxisomal proliferator-activated receptor γ (PPAR-γ)] or increased the activity of selected phase I and phase II detoxification enzymes in rat tissues. The cloned rat CMO2 gene was 92 and 82% homologous to the mouse and human CMO2 nucleotide sequence, respectively. The relative abundance of CMO1, CMO2, and PPAR-γ were differentially expressed among rat tissues. CMO1 and PPAR-γ expression were decreased in the kidney and adrenal with lycopene intake (P < 0.05), whereas CMO2 expression was reduced only in the kidney. Lycopene did not alter hepatic phase I activity, but hepatic quinone reductase activity increased after 3 and 7 d of lycopene feeding (P < 0.05). Lycopene intake decreased a PPAR-γ target gene, fatty acid binding protein 3 (FABP3), in the kidney and adrenal (P < 0.05). Thus, these data show that although the intake of 0.25 g lycopene/kg diet does not induce hepatic P450 detoxification enzymes, lycopene feeding alters CMO1, PPAR-γ, and FABP3 mRNA expression in selected rat tissues with a moderate effect on kidney CMO2 expression. These data suggest that lycopene may play an important role in the modulation of β-carotene, retinoid, and/or lipid metabolism.
AB - In vitro studies have suggested that lycopene is an efficient substrate for carotenoid 9′10′-monooxygenase II (CMO2) but an inhibitor of carotenoid 15,15′-monooxygenase I (CMO1). The objectives of this study were to clone the rat CMO2 gene, determine whether feeding lycopene for different lengths of time (3-37 d) altered the expression of genes related to carotenoid cleavage [CMO1, CMO2 and peroxisomal proliferator-activated receptor γ (PPAR-γ)] or increased the activity of selected phase I and phase II detoxification enzymes in rat tissues. The cloned rat CMO2 gene was 92 and 82% homologous to the mouse and human CMO2 nucleotide sequence, respectively. The relative abundance of CMO1, CMO2, and PPAR-γ were differentially expressed among rat tissues. CMO1 and PPAR-γ expression were decreased in the kidney and adrenal with lycopene intake (P < 0.05), whereas CMO2 expression was reduced only in the kidney. Lycopene did not alter hepatic phase I activity, but hepatic quinone reductase activity increased after 3 and 7 d of lycopene feeding (P < 0.05). Lycopene intake decreased a PPAR-γ target gene, fatty acid binding protein 3 (FABP3), in the kidney and adrenal (P < 0.05). Thus, these data show that although the intake of 0.25 g lycopene/kg diet does not induce hepatic P450 detoxification enzymes, lycopene feeding alters CMO1, PPAR-γ, and FABP3 mRNA expression in selected rat tissues with a moderate effect on kidney CMO2 expression. These data suggest that lycopene may play an important role in the modulation of β-carotene, retinoid, and/or lipid metabolism.
KW - 10′-monooxygenase
KW - Lycopene
KW - Prostate
KW - Rats
KW - β-carotene 15,15′-monooxygenase
KW - β-carotene 9′
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U2 - 10.1093/jn/136.4.932
DO - 10.1093/jn/136.4.932
M3 - Article
C2 - 16549453
AN - SCOPUS:33645509662
SN - 0022-3166
VL - 136
SP - 932
EP - 938
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 4
ER -