TY - JOUR
T1 - Dietary licorice root supplementation reduces diet-induced weight gain, lipid deposition, and hepatic steatosis in ovariectomized mice without stimulating reproductive tissues and mammary gland
AU - Madak-Erdogan, Zeynep
AU - Gong, Ping
AU - Zhao, Yiru Chen
AU - Xu, Liwen
AU - Wrobel, Kinga U.
AU - Hartman, James A.
AU - Wang, Michelle
AU - Cam, Anthony
AU - Iwaniec, Urszula T.
AU - Turner, Russell T.
AU - Twaddle, Nathan C.
AU - Doerge, Daniel R.
AU - Khan, Ikhlas A.
AU - Katzenellenbogen, John A.
AU - Katzenellenbogen, Benita S.
AU - Helferich, William G.
N1 - Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Scope: We studied the impact of dietary supplementation with licorice root components on diet-induced obesity, fat accumulation, and hepatic steatosis in ovariectomized C57BL/6 mice as a menopause model. Materials and methods: We evaluated the molecular and physiological effects of dietary licorice root administered to ovariectomized C57BL/6 mice as root powder (LRP), extracts (LRE), or isolated isoliquiritigenin (ILQ) on reproductive (uterus and mammary gland) and nonreproductive tissues important in regulating metabolism (liver, perigonadal, perirenal, mesenteric, and subcutaneous fat). Quantitative outcome measures including body weight, fat distribution (magnetic resonance imaging), food consumption, bone density and weight (Dual-energy X-ray absorptiometry), and gene expression were assessed by the degree of restoration to the preovariectomized health state. We characterized histological (H&E and oil red O staining) and molecular properties (expression of certain disease markers) of these tissues, and correlated these with metabolic phenotype as well as blood levels of bioactives. Conclusion: Although LRE and ILQ provided some benefit, LRP was the most effective in reducing body weight gain, overall fat deposition, liver steatosis, and expression of hepatic lipid synthesis genes following ovariectomy. Our data demonstrate that licorice root provided improvement of multiple metabolic parameters under conditions of low estrogen and high-fat diets without stimulating reproductive tissues.
AB - Scope: We studied the impact of dietary supplementation with licorice root components on diet-induced obesity, fat accumulation, and hepatic steatosis in ovariectomized C57BL/6 mice as a menopause model. Materials and methods: We evaluated the molecular and physiological effects of dietary licorice root administered to ovariectomized C57BL/6 mice as root powder (LRP), extracts (LRE), or isolated isoliquiritigenin (ILQ) on reproductive (uterus and mammary gland) and nonreproductive tissues important in regulating metabolism (liver, perigonadal, perirenal, mesenteric, and subcutaneous fat). Quantitative outcome measures including body weight, fat distribution (magnetic resonance imaging), food consumption, bone density and weight (Dual-energy X-ray absorptiometry), and gene expression were assessed by the degree of restoration to the preovariectomized health state. We characterized histological (H&E and oil red O staining) and molecular properties (expression of certain disease markers) of these tissues, and correlated these with metabolic phenotype as well as blood levels of bioactives. Conclusion: Although LRE and ILQ provided some benefit, LRP was the most effective in reducing body weight gain, overall fat deposition, liver steatosis, and expression of hepatic lipid synthesis genes following ovariectomy. Our data demonstrate that licorice root provided improvement of multiple metabolic parameters under conditions of low estrogen and high-fat diets without stimulating reproductive tissues.
KW - Botanical estrogens
KW - Dietary supplements
KW - Estrogen receptor
KW - Licorice root
KW - Menopause
KW - Metabolism
UR - http://www.scopus.com/inward/record.url?scp=84948176972&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84948176972&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201500445
DO - 10.1002/mnfr.201500445
M3 - Article
C2 - 26555669
AN - SCOPUS:84948176972
SN - 1613-4125
VL - 60
SP - 369
EP - 380
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 2
ER -