Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency

Ousseynou Sarr, George W. Payne, Barbora Hucik, Salma Abdelmagid, Manabu T Nakamura, David W.L. Ma, David M. Mutch

Research output: Contribution to journalArticle

Abstract

Fatty acid desaturase 2 (Fads2) encodes the delta-6 desaturase (D6D) enzyme, which is rate-limiting for the endogenous production of omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). Numerous studies have reported the cardiometabolic health benefits of omega-3 LC-PUFA. Humans carrying genetic variants in the FADS2 gene have reduced levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), as well as oxylipins, in blood, erythrocytes and white adipose tissue (WAT). Similar findings have been reported in whole-body Fads2−/− mice fed a diet deficient in omega-3 LC-PUFA. The objective of this study was to determine if a diet containing EPA and DHA would prevent the deficiencies in WAT lipid profiles seen in Fads2−/− mice fed a diet containing only ALA. Male C57BL/6 J Fads2−/− and wild type (WT) mice were fed a low fat (7% w/w) diet for 9 weeks containing either flaxseed oil + ARASCO (FD, containing~53% ALA) or menhaden oil (MD, containing~14% EPA and 10% DHA). Fads2−/− mice fed an ALA-enriched diet had reduced body weight, little-to-no omega-3 LC-PUFA and a near complete loss of all omega-3 derived oxylipins in both epididymal and inguinal WAT (P<.05) compared to their WT counterparts, as well as altered expression of key regulators of the fatty acid desaturase pathway. However, Fads2−/− mice fed a diet containing EPA and DHA prevented most of these changes. This study provides evidence that a diet containing EPA and DHA provides a nutritional strategy to prevent alterations in WAT lipid content caused by reduced D6D activity.

LanguageEnglish (US)
Pages140-149
Number of pages10
JournalJournal of Nutritional Biochemistry
Volume63
DOIs
StatePublished - Jan 1 2019

Fingerprint

Oxylipins
Fatty Acid Desaturases
White Adipose Tissue
Eicosapentaenoic Acid
Docosahexaenoic Acids
Nutrition
Tissue
Diet
Unsaturated Fatty Acids
Linoleoyl-CoA Desaturase
Linseed Oil
Lipids
Groin
Medical Genetics
Insurance Benefits
Blood
Genes
Erythrocytes
Fats
Body Weight

Keywords

  • Delta-6 desaturase
  • Eicosanoids
  • Epididymal
  • Fatty acids
  • Low-fat diet

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency. / Sarr, Ousseynou; Payne, George W.; Hucik, Barbora; Abdelmagid, Salma; Nakamura, Manabu T; Ma, David W.L.; Mutch, David M.

In: Journal of Nutritional Biochemistry, Vol. 63, 01.01.2019, p. 140-149.

Research output: Contribution to journalArticle

Sarr, Ousseynou ; Payne, George W. ; Hucik, Barbora ; Abdelmagid, Salma ; Nakamura, Manabu T ; Ma, David W.L. ; Mutch, David M. / Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency. In: Journal of Nutritional Biochemistry. 2019 ; Vol. 63. pp. 140-149.
@article{fa68eb1f416242fb80c931d21f960073,
title = "Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency",
abstract = "Fatty acid desaturase 2 (Fads2) encodes the delta-6 desaturase (D6D) enzyme, which is rate-limiting for the endogenous production of omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). Numerous studies have reported the cardiometabolic health benefits of omega-3 LC-PUFA. Humans carrying genetic variants in the FADS2 gene have reduced levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), as well as oxylipins, in blood, erythrocytes and white adipose tissue (WAT). Similar findings have been reported in whole-body Fads2−/− mice fed a diet deficient in omega-3 LC-PUFA. The objective of this study was to determine if a diet containing EPA and DHA would prevent the deficiencies in WAT lipid profiles seen in Fads2−/− mice fed a diet containing only ALA. Male C57BL/6 J Fads2−/− and wild type (WT) mice were fed a low fat (7{\%} w/w) diet for 9 weeks containing either flaxseed oil + ARASCO (FD, containing~53{\%} ALA) or menhaden oil (MD, containing~14{\%} EPA and 10{\%} DHA). Fads2−/− mice fed an ALA-enriched diet had reduced body weight, little-to-no omega-3 LC-PUFA and a near complete loss of all omega-3 derived oxylipins in both epididymal and inguinal WAT (P<.05) compared to their WT counterparts, as well as altered expression of key regulators of the fatty acid desaturase pathway. However, Fads2−/− mice fed a diet containing EPA and DHA prevented most of these changes. This study provides evidence that a diet containing EPA and DHA provides a nutritional strategy to prevent alterations in WAT lipid content caused by reduced D6D activity.",
keywords = "Delta-6 desaturase, Eicosanoids, Epididymal, Fatty acids, Low-fat diet",
author = "Ousseynou Sarr and Payne, {George W.} and Barbora Hucik and Salma Abdelmagid and Nakamura, {Manabu T} and Ma, {David W.L.} and Mutch, {David M.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.jnutbio.2018.09.017",
language = "English (US)",
volume = "63",
pages = "140--149",
journal = "Journal of Nutritional Biochemistry",
issn = "0955-2863",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Dietary EPA and DHA prevent changes in white adipose tissue omega-3 PUFA and oxylipin content associated with a Fads2 deficiency

AU - Sarr, Ousseynou

AU - Payne, George W.

AU - Hucik, Barbora

AU - Abdelmagid, Salma

AU - Nakamura, Manabu T

AU - Ma, David W.L.

AU - Mutch, David M.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Fatty acid desaturase 2 (Fads2) encodes the delta-6 desaturase (D6D) enzyme, which is rate-limiting for the endogenous production of omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). Numerous studies have reported the cardiometabolic health benefits of omega-3 LC-PUFA. Humans carrying genetic variants in the FADS2 gene have reduced levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), as well as oxylipins, in blood, erythrocytes and white adipose tissue (WAT). Similar findings have been reported in whole-body Fads2−/− mice fed a diet deficient in omega-3 LC-PUFA. The objective of this study was to determine if a diet containing EPA and DHA would prevent the deficiencies in WAT lipid profiles seen in Fads2−/− mice fed a diet containing only ALA. Male C57BL/6 J Fads2−/− and wild type (WT) mice were fed a low fat (7% w/w) diet for 9 weeks containing either flaxseed oil + ARASCO (FD, containing~53% ALA) or menhaden oil (MD, containing~14% EPA and 10% DHA). Fads2−/− mice fed an ALA-enriched diet had reduced body weight, little-to-no omega-3 LC-PUFA and a near complete loss of all omega-3 derived oxylipins in both epididymal and inguinal WAT (P<.05) compared to their WT counterparts, as well as altered expression of key regulators of the fatty acid desaturase pathway. However, Fads2−/− mice fed a diet containing EPA and DHA prevented most of these changes. This study provides evidence that a diet containing EPA and DHA provides a nutritional strategy to prevent alterations in WAT lipid content caused by reduced D6D activity.

AB - Fatty acid desaturase 2 (Fads2) encodes the delta-6 desaturase (D6D) enzyme, which is rate-limiting for the endogenous production of omega-3 long-chain polyunsaturated fatty acids (LC-PUFA). Numerous studies have reported the cardiometabolic health benefits of omega-3 LC-PUFA. Humans carrying genetic variants in the FADS2 gene have reduced levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), as well as oxylipins, in blood, erythrocytes and white adipose tissue (WAT). Similar findings have been reported in whole-body Fads2−/− mice fed a diet deficient in omega-3 LC-PUFA. The objective of this study was to determine if a diet containing EPA and DHA would prevent the deficiencies in WAT lipid profiles seen in Fads2−/− mice fed a diet containing only ALA. Male C57BL/6 J Fads2−/− and wild type (WT) mice were fed a low fat (7% w/w) diet for 9 weeks containing either flaxseed oil + ARASCO (FD, containing~53% ALA) or menhaden oil (MD, containing~14% EPA and 10% DHA). Fads2−/− mice fed an ALA-enriched diet had reduced body weight, little-to-no omega-3 LC-PUFA and a near complete loss of all omega-3 derived oxylipins in both epididymal and inguinal WAT (P<.05) compared to their WT counterparts, as well as altered expression of key regulators of the fatty acid desaturase pathway. However, Fads2−/− mice fed a diet containing EPA and DHA prevented most of these changes. This study provides evidence that a diet containing EPA and DHA provides a nutritional strategy to prevent alterations in WAT lipid content caused by reduced D6D activity.

KW - Delta-6 desaturase

KW - Eicosanoids

KW - Epididymal

KW - Fatty acids

KW - Low-fat diet

UR - http://www.scopus.com/inward/record.url?scp=85055259624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055259624&partnerID=8YFLogxK

U2 - 10.1016/j.jnutbio.2018.09.017

DO - 10.1016/j.jnutbio.2018.09.017

M3 - Article

VL - 63

SP - 140

EP - 149

JO - Journal of Nutritional Biochemistry

T2 - Journal of Nutritional Biochemistry

JF - Journal of Nutritional Biochemistry

SN - 0955-2863

ER -