Diet and exercise orthogonally alter the gut microbiome and reveal independent associations with anxiety and cognition

Silvia S. Kang, Patricio R. Jeraldo, Aishe Kurti, Margret E.Berg Miller, Marc D. Cook, Keith Whitlock, Nigel D Goldenfeld, Jeffrey A Woods, Bryan A White, Nicholas Chia, John D. Fryer

Research output: Contribution to journalArticle

Abstract

Background: The ingestion of a high-fat diet (HFD) and the resulting obese state can exert a multitude of stressors on the individual including anxiety and cognitive dysfunction. Though many studies have shown that exercise can alleviate the negative consequences of a HFD using metabolic readouts such as insulin and glucose, a paucity of well-controlled rodent studies have been published on HFD and exercise interactions with regard to behavioral outcomes. This is a critical issue since some individuals assume that HFD-induced behavioral problems such as anxiety and cognitive dysfunction can simply be exercised away. To investigate this, we analyzed mice fed a normal diet (ND), ND with exercise, HFD diet, or HFD with exercise. Results: We found that mice on a HFD had robust anxiety phenotypes but this was not rescued by exercise. Conversely, exercise increased cognitive abilities but this was not impacted by the HFD. Given the importance of the gut microbiome in shaping the host state, we used 16S rRNA hypervariable tag sequencing to profile our cohorts and found that HFD massively reshaped the gut microbial community in agreement with numerous published studies. However, exercise alone also caused massive shifts in the gut microbiome at nearly the same magnitude as diet but these changes were surprisingly orthogonal. Additionally, specific bacterial abundances were directly proportional to measures of anxiety or cognition. Conclusions: Thus, behavioral domains and the gut microbiome are both impacted by diet and exercise but in unrelated ways. These data have important implications for obesity research aimed at modifications of the gut microbiome and suggest that specific gut microbes could be used as a biomarker for anxiety or cognition or perhaps even targeted for therapy.

Original languageEnglish (US)
Article number36
JournalMolecular Neurodegeneration
Volume9
Issue number1
DOIs
StatePublished - Sep 13 2014

Fingerprint

High Fat Diet
Cognition
Anxiety
Diet
Gastrointestinal Microbiome
Aptitude
Rodentia
Obesity
Eating
Biomarkers
Insulin
Phenotype
Glucose
Research

Keywords

  • Anxiety
  • Cognition
  • Diet
  • Exercise
  • Gut-brain axis
  • Microbiome
  • Neuroscience

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Kang, S. S., Jeraldo, P. R., Kurti, A., Miller, M. E. B., Cook, M. D., Whitlock, K., ... Fryer, J. D. (2014). Diet and exercise orthogonally alter the gut microbiome and reveal independent associations with anxiety and cognition. Molecular Neurodegeneration, 9(1), [36]. https://doi.org/10.1186/1750-1326-9-36

Diet and exercise orthogonally alter the gut microbiome and reveal independent associations with anxiety and cognition. / Kang, Silvia S.; Jeraldo, Patricio R.; Kurti, Aishe; Miller, Margret E.Berg; Cook, Marc D.; Whitlock, Keith; Goldenfeld, Nigel D; Woods, Jeffrey A; White, Bryan A; Chia, Nicholas; Fryer, John D.

In: Molecular Neurodegeneration, Vol. 9, No. 1, 36, 13.09.2014.

Research output: Contribution to journalArticle

Kang, Silvia S. ; Jeraldo, Patricio R. ; Kurti, Aishe ; Miller, Margret E.Berg ; Cook, Marc D. ; Whitlock, Keith ; Goldenfeld, Nigel D ; Woods, Jeffrey A ; White, Bryan A ; Chia, Nicholas ; Fryer, John D. / Diet and exercise orthogonally alter the gut microbiome and reveal independent associations with anxiety and cognition. In: Molecular Neurodegeneration. 2014 ; Vol. 9, No. 1.
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