Diaporthe seed decay of soybean [Glycine max (L.) Merr.] is endemic in the united states, but new fungi are involved

Kristina Petrović, Demetra Skaltsas, Lisa A. Castlebury, Brian Kontz, Tom W. Allen, Martin I. Chilvers, Nancy Gregory, Heather M. Kelly, Alyssa M. Koehler, Nathan M. Kleczewski, Daren S. Mueller, Paul P. Price, Damon L. Smith, Febina M. Mathew

Research output: Contribution to journalArticlepeer-review


Diaporthe seed decay can compromise seed quality in soybean [Glycine max (L.) Merr.] in the warm and humid production areas of the United States during crop maturation. In the current study, 45 isolates of Diaporthe were recovered from seed sampled from soybean fields affected by Diaporthe-associated diseases in eight U.S. states in 2017. The isolates obtained belonged to 10 species of Diaporthe based on morphology and phylogenetic analyses of the internal transcribed spacer, partial translation elongation factor 1-a, and b-tubulin gene sequences. The associated species included D. aspalathi, D. caulivora, D. kongii, D. longicolla, D. sojae, D. ueckerae, D. unshiuensis, and three novel fungi, D. bacilloides, D. flavescens, and D. insulistroma. One isolate each of the 10 species was examined for pathogenicity on seed of cultivar Sava under controlled conditions. Seven days postinoculation, significant differences in the percentages of decayed seeds and seedling necrosis were observed among the isolates and the noninoculated control (P < 0.0001). While the isolates of D. bacilloides, D. longicolla, and D. ueckerae caused a significantly greater percentage of decayed seeds (P < 0.0001), the isolate of D. aspalathi caused the greatest seedling necrosis (P < 0.0001). The observation of new fungi causing Diaporthe seed decay suggests the need for a more comprehensive survey in U.S. soybean producing areas since members of the genus Diaporthe appear to form a complex that causes seed decay.

Original languageEnglish (US)
JournalPlant disease
Issue number6
StatePublished - Jun 2021


  • Diaporthe
  • Field crops
  • Fungi
  • New taxa
  • Oilseeds and legumes
  • Pathogen diversity
  • Phomopsis seed decay
  • Soybean
  • Taxonomy

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science


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