@article{dbe55f509c41469abd8124711ed38733,
title = "Diagnostic accuracy of optical coherence tomography for assessing surgical margins of canine soft tissue sarcomas in observers of different specialties",
abstract = "Objective: To determine the diagnostic accuracy of optical coherence tomography (OCT) to assess surgical margins of canine soft tissue sarcoma (STS) and determine the influence of observer specialty and training. Study design: Blinded clinical prospective study. Animals: Twenty-five dogs undergoing surgical excision of STS. Methods: In vivo and ex vivo surgical margins were imaged with OCT after tumor resection. Representative images and videos were used to generate a training presentation and data sets. These were completed by 16 observers of four specialties (surgery, radiology, pathology, and OCT researchers). Images and videos from data sets were classified as cancerous or noncancerous. Results: The overall sensitivity and specificity were 88.2% and 92.8%, respectively, for in vivo tissues and 82.5% and 93.3%, respectively, for ex vivo specimens. The overall accurate classification for all specimens was 91.4% in vivo and 89.5% ex vivo. There was no difference in accuracy of interpretation of OCT imaging by observers of different specialties or experience levels. Conclusion: Use of OCT to accurately assess surgical margins after STS excision was associated with a high sensitivity and specificity among various specialties. Personnel of all specialties and experience levels could effectively be trained to interpret OCT imaging. Clinical significance: Optical coherence tomography can be used by personnel of different specialty experience levels and from various specialties to accurately identify canine STS in vivo and ex vivo after a short training session. These encouraging results provide evidence to justify further research to assess the ability of OCT to provide real-time assessments of surgical margins and its applicability to other neoplasms.",
author = "Dornbusch, {Josephine A.} and Laura Selmic and Huang, {Pin Chieh} and Samuelson, {Jonathan P.} and McLaughlin, {Eric M.} and Wavreille, {Vincent A.} and Ogden, {Jessica A.} and Brittany Abrams and Alex Kalamaras and Eric Green and Hostnik, {Eric T.} and Lincoln Every and Fuerst, {Jason A.} and Ryan Jennings and Christopher Premanandan and Lorbach, {Joshua N.} and Linn, {Sarah C.} and Aneesh Alex and Sorrells, {Janet E.} and Lingxiao Yang and Boppart, {Stephen A.}",
note = "Funding Information: Dornbusch JA, DVM: Data collection, data cleaning and data interpretation, drafting the manuscript, and approval of the final manuscript; Selmic LE, BVetMed (Hons), MPH DACVS-SA, DECVS: Conceptualization and design of the study, data cleaning and data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Huang P-C, BS, MS: Imaging data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Samuelson JP, DVM, MS, DACVP: Pathology data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; McLaughlin EM, MS: Data analysis, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Wavreille VA, DVM, DACVS-SA: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Ogden JA, DVM, DACVS-SA: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Abrams B, DVM: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Kalamaras A, DVM: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Green E, DVM, DACVR: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Hostnik ET, DVM, DACVR, DACVR-EDI: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Every L, DVM, DACVR: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Fuerst JA, DVM, MS, DACVR: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Jennings R, DVM, PhD, DACVP: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Premanandan C, DVM, PhD, DACVP, DACT: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Lorbach JN, DVM: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Linn SC, DVM: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Alex A, PhD: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Sorrells JE, BS: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Yang L, BS: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript; Boppart SA, MD, PhD: Data collection, data interpretation, drafting and revising the manuscript, and approval of the final manuscript. The authors thank Jennifer Reagan DVM DACVS-SA for her assistance in designing the study, Guillermo Monroy PhD for his assistance in modifying the custom build OCT system, and Jianfeng Wang PhD for his assistance in building the OCT training PowerPoint. This project was supported by the AKC Health Foundation (Grant No. 2204-T), and in part by the National Institutes of Health (Grant No. R01 CA213149). The contents of this publication are solely the responsibility of the authors and do not necessarily represent the view of the foundation. The project described was supported by Award Number UL1TR001070 from the National Center for Advancing Translational Sciences. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Advancing Translational Sciences or the National Institutes of Health. Funding Information: This project was supported by the AKC Health Foundation (Grant No. 2204‐T), and in part by the National Institutes of Health (Grant No. R01 CA213149). The contents of this publication are solely the responsibility of the authors and do not necessarily represent the view of the foundation. The project described was supported by Award Number UL1TR001070 from the National Center for Advancing Translational Sciences. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Advancing Translational Sciences or the National Institutes of Health.",
year = "2021",
month = jan,
doi = "10.1111/vsu.13510",
language = "English (US)",
volume = "50",
pages = "111--120",
journal = "Veterinary Surgery",
issn = "0161-3499",
publisher = "Wiley-Blackwell",
number = "1",
}