Developments in in vitro technologies for swine embryo production

Matthew B. Wheeler, Sherrie G. Clark, David J. Beebe

Research output: Contribution to journalReview articlepeer-review

Abstract

Several modifications have been made to in vitro production (IVP) systems to allow more efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 30 years, the overall blastocyst production rate remains low. The low blastocyst rate is due to several factors, including polyspermic oocyte penetration, low rate of male pronucleus formation and less than optimal in vitro culture systems. These conditions are all inherent problems in porcine IVP and many of the mechanisms involved remain unknown. Considerable research has examined culture medium and the techniques used during the various stages of in vitro production. However, changes to the physical culture system used during IVF have remained unchanged until recently. The present paper will summarise selected developments in fertilisation and embryo culture media composition and focus on the development of modified equipment to improve the conditions used during the IVP of porcine oocytes and embryos.

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalReproduction, Fertility and Development
Volume16
Issue number1-2
DOIs
StatePublished - Jan 1 2004

Keywords

  • In vitro production
  • Microchannel
  • Microfluidics
  • Porcine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology

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