Developments in in vitro technologies for swine embryo production

Matthew B. Wheeler, Sherrie G. Clark, David J. Beebe

Research output: Contribution to journalReview article

Abstract

Several modifications have been made to in vitro production (IVP) systems to allow more efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 30 years, the overall blastocyst production rate remains low. The low blastocyst rate is due to several factors, including polyspermic oocyte penetration, low rate of male pronucleus formation and less than optimal in vitro culture systems. These conditions are all inherent problems in porcine IVP and many of the mechanisms involved remain unknown. Considerable research has examined culture medium and the techniques used during the various stages of in vitro production. However, changes to the physical culture system used during IVF have remained unchanged until recently. The present paper will summarise selected developments in fertilisation and embryo culture media composition and focus on the development of modified equipment to improve the conditions used during the IVP of porcine oocytes and embryos.

Original languageEnglish (US)
Pages (from-to)15-25
Number of pages11
JournalReproduction, Fertility and Development
Volume16
Issue number1-2
StatePublished - 2004

Fingerprint

embryo (animal)
Swine
Embryonic Structures
Technology
blastocyst
swine
oocytes
culture media
pronucleus
embryo culture
Blastocyst
in vitro culture
Oocytes
Culture Media
production technology
Culture Techniques
Fertilization
In Vitro Techniques
Equipment and Supplies
methodology

Keywords

  • In vitro production
  • Microchannel
  • Microfluidics
  • Porcine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology

Cite this

Developments in in vitro technologies for swine embryo production. / Wheeler, Matthew B.; Clark, Sherrie G.; Beebe, David J.

In: Reproduction, Fertility and Development, Vol. 16, No. 1-2, 2004, p. 15-25.

Research output: Contribution to journalReview article

Wheeler, Matthew B. ; Clark, Sherrie G. ; Beebe, David J. / Developments in in vitro technologies for swine embryo production. In: Reproduction, Fertility and Development. 2004 ; Vol. 16, No. 1-2. pp. 15-25.
@article{965047d4bef546da800cd780701617f5,
title = "Developments in in vitro technologies for swine embryo production",
abstract = "Several modifications have been made to in vitro production (IVP) systems to allow more efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 30 years, the overall blastocyst production rate remains low. The low blastocyst rate is due to several factors, including polyspermic oocyte penetration, low rate of male pronucleus formation and less than optimal in vitro culture systems. These conditions are all inherent problems in porcine IVP and many of the mechanisms involved remain unknown. Considerable research has examined culture medium and the techniques used during the various stages of in vitro production. However, changes to the physical culture system used during IVF have remained unchanged until recently. The present paper will summarise selected developments in fertilisation and embryo culture media composition and focus on the development of modified equipment to improve the conditions used during the IVP of porcine oocytes and embryos.",
keywords = "In vitro production, Microchannel, Microfluidics, Porcine",
author = "Wheeler, {Matthew B.} and Clark, {Sherrie G.} and Beebe, {David J.}",
year = "2004",
language = "English (US)",
volume = "16",
pages = "15--25",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "1-2",

}

TY - JOUR

T1 - Developments in in vitro technologies for swine embryo production

AU - Wheeler, Matthew B.

AU - Clark, Sherrie G.

AU - Beebe, David J.

PY - 2004

Y1 - 2004

N2 - Several modifications have been made to in vitro production (IVP) systems to allow more efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 30 years, the overall blastocyst production rate remains low. The low blastocyst rate is due to several factors, including polyspermic oocyte penetration, low rate of male pronucleus formation and less than optimal in vitro culture systems. These conditions are all inherent problems in porcine IVP and many of the mechanisms involved remain unknown. Considerable research has examined culture medium and the techniques used during the various stages of in vitro production. However, changes to the physical culture system used during IVF have remained unchanged until recently. The present paper will summarise selected developments in fertilisation and embryo culture media composition and focus on the development of modified equipment to improve the conditions used during the IVP of porcine oocytes and embryos.

AB - Several modifications have been made to in vitro production (IVP) systems to allow more efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 30 years, the overall blastocyst production rate remains low. The low blastocyst rate is due to several factors, including polyspermic oocyte penetration, low rate of male pronucleus formation and less than optimal in vitro culture systems. These conditions are all inherent problems in porcine IVP and many of the mechanisms involved remain unknown. Considerable research has examined culture medium and the techniques used during the various stages of in vitro production. However, changes to the physical culture system used during IVF have remained unchanged until recently. The present paper will summarise selected developments in fertilisation and embryo culture media composition and focus on the development of modified equipment to improve the conditions used during the IVP of porcine oocytes and embryos.

KW - In vitro production

KW - Microchannel

KW - Microfluidics

KW - Porcine

UR - http://www.scopus.com/inward/record.url?scp=0346121523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346121523&partnerID=8YFLogxK

M3 - Review article

C2 - 14972099

AN - SCOPUS:0346121523

VL - 16

SP - 15

EP - 25

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 1-2

ER -