Developmental regulation of a Turkey intestinal peptide transporter (PepT1)

L. Van, Y. X. Pan, J. R. Bloomquist, K. E. Webb, E. A. Wong

Research output: Contribution to journalArticlepeer-review


A cDNA encoding a turkey intestinal peptide transporter, tPepT1, was isolated from a turkey small intestinal cDNA library. The tPepT1 cDNA encodes a 714-amino acid protein with 12 predicted transmembrane domains. The amino acid sequence of tPepT1 is 94.3% identical to chicken PepT1 and approximately 60% identical to PepT1 from rat, sheep, rabbit, and human. Using a 2-electrode voltage-clamp technique in Xenopus oocytes expressing tPepT1, Gly-Sar transport was pH dependent but was independent of Na+ and K+. For the dipeptides Gly-Sar and Met-Met, the evoked inward currents indicated that the transporter was saturable and had high affinity (0.69 ± 0.14 mM and 0.23 ± 0.04 mM, respectively) for these substrates. However, transport of the tetrapeptide, Met-Gly-Met-Met, exhibited apparent substrate inhibition at high substrate concentrations. To study developmental regulation of PepT1 mRNA in turkey embryos, embryos (6 males and 6 females) were sampled daily from 5 d before hatch to the day of hatch (d 0). The abundance of PepT1 mRNA in the small intestine was quantified densitometrically from Northern blots after hybridization with full-length tPepT1 cDNA as probe. A 3.2-fold increase in PepT1 mRNA was observed in intestinal tissue from 5 d before hatch to d 0. This increase in PepT1 mRNA abundance indicates that the PepT1 gene is developmentally regulated and that there may be an important role for PepT1 in the neonatal poult.

Original languageEnglish (US)
Pages (from-to)75-82
Number of pages8
JournalPoultry science
Issue number1
StatePublished - Jan 2005
Externally publishedYes


  • Developmental regulation
  • Embryo
  • PepT1
  • Peptide transporter
  • Turkey

ASJC Scopus subject areas

  • Animal Science and Zoology


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