Developmental genetics of teleosts: A biochemical analysis of lake chubsucker ontogeny

James B. Shaklee, Michael J. Champion, Gregory S. Whitt

Research output: Contribution to journalArticlepeer-review


Developing embryos of the lake chubsucker, Erimyzon sucetta, were analyzed with regard to both gross morphological changes and specific enzymatic changes from the unfertilized egg stage until some 3 weeks posthatching. Total activities of three enzymes-lactate dehydrogenase, glucose-6-phosphate dehydrogenase, and isocitrate dehydrogenase-were determined throughout the course of development. Each of these different enzymes exhibited a different pattern of change during ontogeny. Electrophoretic analysis of qualitative changes in isozyme patterns was accomplished for these three enzymes and for α-amylase, glucosephosphate isomerase, mannosephosphate isomerase, creatine kinase, esterase, glutamate dehydrogenase, alkaline phosphatase, aspartate aminotransferase, malate dehydrogenase, hexose diphosphatase, phosphoglucomutase, and phosphogluconate dehydrogenase. Many of the enzyme systems investigated exhibited rich patterns of ontogenetic change, while a few remained relatively unchanged throughout the interval studied. Several of the enzymes in particular metabolic pathways exhibited coincident changes suggestive of coordinate control. The appearance of several rather "tissue-specific" isozymes was closely correlated with the morphological and functional differentiation of these particular tissues or organs.

Original languageEnglish (US)
Pages (from-to)356-382
Number of pages27
JournalDevelopmental Biology
Issue number2
StatePublished - Jun 1974

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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