TY - JOUR
T1 - Developmental genetics of teleosts
T2 - A biochemical analysis of lake chubsucker ontogeny
AU - Shaklee, James B.
AU - Champion, Michael J.
AU - Whitt, Gregory S.
N1 - Funding Information:
The authors wish to thank R. Kocher, G. Lewis, J. Tranquilli, and Dr. William F. Childers (Aquatic Biology Section, Illinois Natural History Survey, Urbana, Illinois) for providing the parental lake chubsuckers used in this study. Dr. David L. Stocum (Zoology Department, University of Illinois, Urbana, Illinois) kindly furnished the dissecting microscope and camera used to photograph the developing embryos. We are especially grateful to Dr. William W. Ballard (Dartmouth College, Hanover, New Hampshire) for the use of the unpublished staging of the normal development of the white sucker. We also thank Drs. W. W. Ballard and C. L. Markert (Biology Department, Yale University, New Haven, Connecticut) for their critical reading of the manuscript. This research was supported by NSF GB 16425t o G. S. W.
PY - 1974/6
Y1 - 1974/6
N2 - Developing embryos of the lake chubsucker, Erimyzon sucetta, were analyzed with regard to both gross morphological changes and specific enzymatic changes from the unfertilized egg stage until some 3 weeks posthatching. Total activities of three enzymes-lactate dehydrogenase, glucose-6-phosphate dehydrogenase, and isocitrate dehydrogenase-were determined throughout the course of development. Each of these different enzymes exhibited a different pattern of change during ontogeny. Electrophoretic analysis of qualitative changes in isozyme patterns was accomplished for these three enzymes and for α-amylase, glucosephosphate isomerase, mannosephosphate isomerase, creatine kinase, esterase, glutamate dehydrogenase, alkaline phosphatase, aspartate aminotransferase, malate dehydrogenase, hexose diphosphatase, phosphoglucomutase, and phosphogluconate dehydrogenase. Many of the enzyme systems investigated exhibited rich patterns of ontogenetic change, while a few remained relatively unchanged throughout the interval studied. Several of the enzymes in particular metabolic pathways exhibited coincident changes suggestive of coordinate control. The appearance of several rather "tissue-specific" isozymes was closely correlated with the morphological and functional differentiation of these particular tissues or organs.
AB - Developing embryos of the lake chubsucker, Erimyzon sucetta, were analyzed with regard to both gross morphological changes and specific enzymatic changes from the unfertilized egg stage until some 3 weeks posthatching. Total activities of three enzymes-lactate dehydrogenase, glucose-6-phosphate dehydrogenase, and isocitrate dehydrogenase-were determined throughout the course of development. Each of these different enzymes exhibited a different pattern of change during ontogeny. Electrophoretic analysis of qualitative changes in isozyme patterns was accomplished for these three enzymes and for α-amylase, glucosephosphate isomerase, mannosephosphate isomerase, creatine kinase, esterase, glutamate dehydrogenase, alkaline phosphatase, aspartate aminotransferase, malate dehydrogenase, hexose diphosphatase, phosphoglucomutase, and phosphogluconate dehydrogenase. Many of the enzyme systems investigated exhibited rich patterns of ontogenetic change, while a few remained relatively unchanged throughout the interval studied. Several of the enzymes in particular metabolic pathways exhibited coincident changes suggestive of coordinate control. The appearance of several rather "tissue-specific" isozymes was closely correlated with the morphological and functional differentiation of these particular tissues or organs.
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U2 - 10.1016/0012-1606(74)90013-X
DO - 10.1016/0012-1606(74)90013-X
M3 - Article
C2 - 4364634
AN - SCOPUS:0016160345
SN - 0012-1606
VL - 38
SP - 356
EP - 382
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -