Continuous and semi-continuous flow bioreactors have been developed for the production of chloroperoxidase, which is secreted by the fungus Caldariomyces fumago. The semi-continuous system facilitated the harvest of mycelium-free supernatant for 200 days from a single inoculum, with enzyme levels greater than 600 mg/l during the first 80 days. The continuous flow bioreactor yielded essentially pure enzyme at the rate of 117 mg/day.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology