Development of microfluidic channels for the culture of mammalian embryos

J. A. Davis, S. Raty, D. T. Eddington, I. K. Glasgow, H. C. Zeringue, M. B. Wheeler, D. J. Beebe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microfluidic channels are explored as a tool for the culture of mammalian embryos. Three different materials are used to construct microfluidic devices for embyro culture: silicon, glass, and polydimethylsiloxane. Practical issues including, loading, localization-visualization and retrieval of the embryos in the microchannels, bubble formation within the device, and development of cleaning and sterilization procedures are discussed and addressed via design iterations. Preliminary data on embryo development within the microchannels have been collected.

Original languageEnglish (US)
Title of host publication1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsAndre Dittmar, David Beebe
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages307-310
Number of pages4
ISBN (Electronic)0780366034, 9780780366039
DOIs
StatePublished - Jan 1 2000
Event1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, MMB 2000 - Lyon, France
Duration: Oct 12 2000Oct 14 2000

Publication series

Name1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Other

Other1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, MMB 2000
CountryFrance
CityLyon
Period10/12/0010/14/00

Fingerprint

Microchannels
Microfluidics
Bubble formation
Polydimethylsiloxane
Cleaning
Visualization
Glass
Silicon

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Davis, J. A., Raty, S., Eddington, D. T., Glasgow, I. K., Zeringue, H. C., Wheeler, M. B., & Beebe, D. J. (2000). Development of microfluidic channels for the culture of mammalian embryos. In A. Dittmar, & D. Beebe (Eds.), 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 307-310). [893793] (1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2000.893793

Development of microfluidic channels for the culture of mammalian embryos. / Davis, J. A.; Raty, S.; Eddington, D. T.; Glasgow, I. K.; Zeringue, H. C.; Wheeler, M. B.; Beebe, D. J.

1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. ed. / Andre Dittmar; David Beebe. Institute of Electrical and Electronics Engineers Inc., 2000. p. 307-310 893793 (1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Davis, JA, Raty, S, Eddington, DT, Glasgow, IK, Zeringue, HC, Wheeler, MB & Beebe, DJ 2000, Development of microfluidic channels for the culture of mammalian embryos. in A Dittmar & D Beebe (eds), 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings., 893793, 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 307-310, 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, MMB 2000, Lyon, France, 10/12/00. https://doi.org/10.1109/MMB.2000.893793
Davis JA, Raty S, Eddington DT, Glasgow IK, Zeringue HC, Wheeler MB et al. Development of microfluidic channels for the culture of mammalian embryos. In Dittmar A, Beebe D, editors, 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2000. p. 307-310. 893793. (1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). https://doi.org/10.1109/MMB.2000.893793
Davis, J. A. ; Raty, S. ; Eddington, D. T. ; Glasgow, I. K. ; Zeringue, H. C. ; Wheeler, M. B. ; Beebe, D. J. / Development of microfluidic channels for the culture of mammalian embryos. 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. editor / Andre Dittmar ; David Beebe. Institute of Electrical and Electronics Engineers Inc., 2000. pp. 307-310 (1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).
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