TY - JOUR
T1 - Development of intrinsically labeled eggs and poultry meat for use in human metabolic research
AU - van Vliet, Stephan
AU - Beals, Joseph W.
AU - Parel, Justin T.
AU - Hanna, Christina D.
AU - Utterback, Pamela L.
AU - Dilger, Anna C.
AU - Ulanov, Alexander V.
AU - Li, Zhong
AU - Paluska, Scott A.
AU - Moore, Daniel R.
AU - Parsons, Carl M.
AU - Burd, Nicholas A.
N1 - Publisher Copyright:
© 2016 American Society for Nutrition.
PY - 2016
Y1 - 2016
N2 - Background: Stable isotope amino acids are regularly used as tracers to examine whole-body and muscle protein metabolism in humans. To accurately assess in vivo dietary protein digestion and absorption kinetics, the amino acid tracer is required to be incorporated within the dietary protein food source (i.e., intrinsically labeled protein). Objective: We assessed the practicality of producing eggs and poultry meat intrinsically labeled with L-[5,5,5-2H3]leucine through noninvasive oral tracer administration. Methods: A specifically formulated diet containing 0.52% leucine was supplemented with 0.3% L-[5,5,5-2H3]leucine and subsequently fed to 3 laying hens (Lohmann LSLWhites) for 55 d. On day 55, the hens were slaughtered and their meat, bones, and organs were harvested to determine tissue labeling. In Expt. 1, 2 healthy youngmen [mean± SEM age: 22 6 1.5 y;mean ± SEM body mass index (BMI; in kg/m2): 23.7 6 0.5] ingested 18 g L-[5,5,5-2H3]leucine-labeled egg protein. In Expt. 2, 2 healthy young men (mean ± SEM age: 20.0 6 0.0 y; mean ± SEM BMI: 26.4 6 3.1) ingested 28 g L-[5,5,5-2H3]leucine-labeled poultry meat protein. Plasma samples (Expts. 1 and 2) andmuscle biopsies (Expt. 1) were collected before and after labeled-food ingestion. Results: High tracer labeling [> 20 mole percent excess (MPE)] in the eggs was obtained after 7 d and maintained throughout the feeding protocol (P < 0.05). Over a 55-d period, ;850 g egg protein (145 eggs)was produced, with amean ± SEMtracer enrichment of 22.060.8MPE.Mean6SEML-[5,5,5-2H3]leucine enrichment in themeat was 9.660.1MPE. In Expts. 1 and 2, the consumption of labeled eggs and poultry meat protein increased plasma L-[5,5,5-2H3]leucine enrichment, withmean ± SEM peak values of 6.7 6 0.1 MPE and 4.0 6 0.9 MPE, respectively. The mean ± SEM 5-h postprandial increase in myofibrillar L-[5,5,5-2H3]leucine enrichment after egg ingestion in healthy young men was 0.051 6 0.008 MPE (Expt. 1). Conclusion: We demonstrated the feasibility of producing intrinsically labeled eggs and poultry meat for use in human metabolic research.
AB - Background: Stable isotope amino acids are regularly used as tracers to examine whole-body and muscle protein metabolism in humans. To accurately assess in vivo dietary protein digestion and absorption kinetics, the amino acid tracer is required to be incorporated within the dietary protein food source (i.e., intrinsically labeled protein). Objective: We assessed the practicality of producing eggs and poultry meat intrinsically labeled with L-[5,5,5-2H3]leucine through noninvasive oral tracer administration. Methods: A specifically formulated diet containing 0.52% leucine was supplemented with 0.3% L-[5,5,5-2H3]leucine and subsequently fed to 3 laying hens (Lohmann LSLWhites) for 55 d. On day 55, the hens were slaughtered and their meat, bones, and organs were harvested to determine tissue labeling. In Expt. 1, 2 healthy youngmen [mean± SEM age: 22 6 1.5 y;mean ± SEM body mass index (BMI; in kg/m2): 23.7 6 0.5] ingested 18 g L-[5,5,5-2H3]leucine-labeled egg protein. In Expt. 2, 2 healthy young men (mean ± SEM age: 20.0 6 0.0 y; mean ± SEM BMI: 26.4 6 3.1) ingested 28 g L-[5,5,5-2H3]leucine-labeled poultry meat protein. Plasma samples (Expts. 1 and 2) andmuscle biopsies (Expt. 1) were collected before and after labeled-food ingestion. Results: High tracer labeling [> 20 mole percent excess (MPE)] in the eggs was obtained after 7 d and maintained throughout the feeding protocol (P < 0.05). Over a 55-d period, ;850 g egg protein (145 eggs)was produced, with amean ± SEMtracer enrichment of 22.060.8MPE.Mean6SEML-[5,5,5-2H3]leucine enrichment in themeat was 9.660.1MPE. In Expts. 1 and 2, the consumption of labeled eggs and poultry meat protein increased plasma L-[5,5,5-2H3]leucine enrichment, withmean ± SEM peak values of 6.7 6 0.1 MPE and 4.0 6 0.9 MPE, respectively. The mean ± SEM 5-h postprandial increase in myofibrillar L-[5,5,5-2H3]leucine enrichment after egg ingestion in healthy young men was 0.051 6 0.008 MPE (Expt. 1). Conclusion: We demonstrated the feasibility of producing intrinsically labeled eggs and poultry meat for use in human metabolic research.
KW - Muscle mass
KW - Muscle protein synthesis
KW - Nutrition
KW - Protein digestion
KW - Stable isotopes
UR - http://www.scopus.com/inward/record.url?scp=84977486756&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84977486756&partnerID=8YFLogxK
U2 - 10.3945/jn.115.228338
DO - 10.3945/jn.115.228338
M3 - Article
C2 - 27281809
AN - SCOPUS:84977486756
SN - 0022-3166
VL - 146
SP - 1428
EP - 1433
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 7
ER -