Development of Host-Orthogonal Genetic Systems for Synthetic Biology

Yang Zhang, Wentao Ding, Zhihui Wang, Huimin Zhao, Shuobo Shi

Research output: Contribution to journalReview articlepeer-review


The construction of a host-orthogonal genetic system can not only minimize the impact of host-specific nuances on fine-tuning of gene expression, but also expand cellular functions such as in vivo continuous evolution of genes based on an error-prone DNA polymerase. It represents an emerging powerful approach for making biology easier to engineer. In this review, the recent advances are described on the design of genetic systems that can be stably inherited in the host cells and are responsible for important biological processes including DNA replication, RNA transcription, protein translation, and gene regulation. Their applications in synthetic biology are summarized and the future challenges and opportunities are discussed in developing such systems.

Original languageEnglish (US)
Article number2000252
JournalAdvanced Biology
Issue number3
StatePublished - Mar 2021


  • host-orthogonal genetic system
  • regulation
  • replication
  • synthetic biology
  • transcription
  • translation

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • Biomedical Engineering
  • Biomaterials


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