Development of genetic methods and construction of a chromosomal glnK ₁ mutant in Methanosarcina mazei strain Gö1

Keyphrases

• Genetic Method 100%
• GlnK 100%
• Plating Efficiency 100%
• Methanosarcina Mazei 100%
• Genetic Construction 100%
• Agar Plate 50%
• Nitrogen Metabolism 50%
• Protein Drug 50%
• Mechanical Stress 50%
• Genetic Manipulation 50%
• Homologous Recombination 50%
• Transcriptional Regulation 50%
• Deletion mutant 50%
• Carbon Source 50%
• PCR Analysis 50%
• Energy Sources 50%
• Trimethylamine 50%
• Growth Defects 50%
• High Resistance 50%
• Methanogenic Archaea 50%
• In the Wild 50%
• Archaeal 50%
• Mutant Strains 50%
• Solid Media 50%
• Quantitative RT-PCR 50%
• Nitrogen Limitation 50%
• Nitrogen Fixation 50%
• Nitrogen Source 50%
• Nitrogen Assimilation 50%
• Spontaneous mutant 50%
• Chromosomal Deletion 50%
• Transformation Method 50%
• Molecular Nitrogen 50%
• Effective Transformation 50%
• G1 Strain 50%
• Transformation Protocol 50%
• Liposome-mediated Transformation 50%
• Top Agar 50%

Biochemistry, Genetics and Molecular Biology

• Genetics 100%
• Agar 100%
• Nitrogen Assimilation 100%
• Methanosarcina mazei 100%
• Mechanical Stress 50%
• Genetic Manipulation 50%
• Wild Type 50%
• Homologous Recombination 50%
• Deletion Mutant 50%
• Methanogenic Archaeon 50%
• Quantitative Reverse Transcription Polymerase Chain Reaction 50%
• Nitrogen Metabolism 50%

Immunology and Microbiology

• Nitrogen Fixation 100%
• Methanosarcina mazei 100%
• Agar Plate 50%
• Nitrogen Metabolism 50%
• Agar 50%
• Wild Type 50%
• Homologous Recombination 50%
• Genetic Manipulation 50%
• Mechanical Stress 50%
• Deletion Mutant 50%
• Methanogenic Archaeon 50%
• Quantitative Reverse Transcription Polymerase Chain Reaction 50%