TY - JOUR
T1 - Development of an Online Tool for Pasteurella multocida Genotyping and Genotypes of Pasteurella multocida From Different Hosts
AU - Peng, Zhong
AU - Liu, Junyang
AU - Liang, Wan
AU - Wang, Fei
AU - Wang, Li
AU - Wang, Xueying
AU - Hua, Lin
AU - Chen, Huanchun
AU - Wilson, Brenda A.
AU - Wang, Jia
AU - Wu, Bin
N1 - This work was supported in part by China Postdoctoral Science Foundation (grant number 2020T130232), the Fundamental Research Funds for the Central Universities of China (grant number 2662018JC034), Guangdong Provincial Key Laboratory of Livestock Disease Prevention (grant number YDWS1901), and Hubei Provincial Key Research & Development Program (grant number 2021BBA085).
We sincerely acknowledge Huazhong Agricultural University College of Informatics for providing the CentOS server and the PubMLST database for the RESTful port for connection.
PY - 2021/12/17
Y1 - 2021/12/17
N2 - Pasteurella multocida is a versatile zoonotic pathogen. Multiple systems have been applied to type P. multocida from different diseases in different hosts. Recently, we found that assigning P. multocida strains by combining their capsular, lipopolysaccharide, and MLST genotypes (marked as capsular: lipopolysaccharide: MLST genotype) could help address the biological characteristics of P. multocida circulation in different hosts. However, there is still lack of a rapid and efficient tool to diagnose P. multocida according to this system. Here, we developed an intelligent genotyping platform PmGT for P. multocida strains according to their whole genome sequences using the web 2.0 technologies. By using PmGT, we determined capsular genotypes, LPS genotypes, and MLST genotypes as well as the main virulence factor genes (VFGs) of P. multocida isolates from different host species based on their whole genome sequences published on NCBI. The results revealed a closer association between the genotypes and pasteurellosis rather than between genotypes and host species. With the advent of high-quality, inexpensive DNA sequencing, PmGT represents a more efficient tool for P. multocida diagnosis in both epidemiological studies and clinical settings.
AB - Pasteurella multocida is a versatile zoonotic pathogen. Multiple systems have been applied to type P. multocida from different diseases in different hosts. Recently, we found that assigning P. multocida strains by combining their capsular, lipopolysaccharide, and MLST genotypes (marked as capsular: lipopolysaccharide: MLST genotype) could help address the biological characteristics of P. multocida circulation in different hosts. However, there is still lack of a rapid and efficient tool to diagnose P. multocida according to this system. Here, we developed an intelligent genotyping platform PmGT for P. multocida strains according to their whole genome sequences using the web 2.0 technologies. By using PmGT, we determined capsular genotypes, LPS genotypes, and MLST genotypes as well as the main virulence factor genes (VFGs) of P. multocida isolates from different host species based on their whole genome sequences published on NCBI. The results revealed a closer association between the genotypes and pasteurellosis rather than between genotypes and host species. With the advent of high-quality, inexpensive DNA sequencing, PmGT represents a more efficient tool for P. multocida diagnosis in both epidemiological studies and clinical settings.
KW - Pasteurella multocida
KW - PmGT
KW - genotypes
KW - genotyping
KW - whole genome sequence
UR - https://www.scopus.com/pages/publications/85122068585
UR - https://www.scopus.com/inward/citedby.url?scp=85122068585&partnerID=8YFLogxK
U2 - 10.3389/fvets.2021.771157
DO - 10.3389/fvets.2021.771157
M3 - Article
C2 - 34977209
AN - SCOPUS:85122068585
SN - 2297-1769
VL - 8
JO - Frontiers in Veterinary Science
JF - Frontiers in Veterinary Science
M1 - 771157
ER -