Abstract
At present serological assays for the detection of “flavescence dorée” (FD), a quarantine disease of grapevine, are not available. The aim of this study was to produce polyclonal antibodies targeting a surface abundant protein of FD and related phytoplasmas, Imp (immunodominant membrane protein), and to verify its use for the detection of FD phytoplasmas in grapevine, other host plant species, and insect vectors. Two antisera were obtained using recombinant Imp proteins from two FD strains. Preliminary results confirmed the specificity and good ELISA performance in the detection of the target in grapevine, clematis and insect vectors tissues.
Original language | English (US) |
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Pages (from-to) | 75-76 |
Number of pages | 2 |
Journal | Phytopathogenic Mollicutes |
Volume | 9 |
Issue number | 1 |
DOIs | |
State | Published - Jun 2019 |
Keywords
- INHS
- Leafhopper
- Grapevine yellows
- Serology
- Phytoplasma
- ELISA
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
- Ecology, Evolution, Behavior and Systematics
- Cell Biology
- Parasitology