Development of an anti-Imp serological assay for the detection of “flavescence dorée” phytoplasmas in grapevine, insect vectors and host plants

Luisa Filippin; Valeria Trivellone; Luciana Galetto; Cristina Marzachí; Vito Elicio; Elisa Angelini

doi:10.5958/2249-4677.2019.00038.0

Development of an anti-Imp serological assay for the detection of “flavescence dorée” phytoplasmas in grapevine, insect vectors and host plants

Luisa Filippin, Valeria Trivellone, Luciana Galetto, Cristina Marzachí, Vito Elicio, Elisa Angelini

Research output: Contribution to journal › Article › peer-review

Abstract

At present serological assays for the detection of “flavescence dorée” (FD), a quarantine disease of grapevine, are not available. The aim of this study was to produce polyclonal antibodies targeting a surface abundant protein of FD and related phytoplasmas, Imp (immunodominant membrane protein), and to verify its use for the detection of FD phytoplasmas in grapevine, other host plant species, and insect vectors. Two antisera were obtained using recombinant Imp proteins from two FD strains. Preliminary results confirmed the specificity and good ELISA performance in the detection of the target in grapevine, clematis and insect vectors tissues.

Original language	English (US)
Pages (from-to)	75-76
Number of pages	2
Journal	Phytopathogenic Mollicutes
Volume	9
Issue number	1
DOIs	https://doi.org/10.5958/2249-4677.2019.00038.0
State	Published - Jun 2019

Keywords

INHS
Leafhopper
Grapevine yellows
Serology
Phytoplasma
ELISA

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases
Ecology, Evolution, Behavior and Systematics
Cell Biology
Parasitology

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Development of an anti-Imp serological assay for the detection of “flavescence dorée” phytoplasmas in grapevine, insect vectors and host plants. / Filippin, Luisa; Trivellone, Valeria; Galetto, Luciana; Marzachí, Cristina; Elicio, Vito; Angelini, Elisa.

In: Phytopathogenic Mollicutes, Vol. 9, No. 1, 06.2019, p. 75-76.

Research output: Contribution to journal › Article › peer-review

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title = "Development of an anti-Imp serological assay for the detection of “flavescence dor{\'e}e” phytoplasmas in grapevine, insect vectors and host plants",

abstract = "At present serological assays for the detection of “flavescence dor{\'e}e” (FD), a quarantine disease of grapevine, are not available. The aim of this study was to produce polyclonal antibodies targeting a surface abundant protein of FD and related phytoplasmas, Imp (immunodominant membrane protein), and to verify its use for the detection of FD phytoplasmas in grapevine, other host plant species, and insect vectors. Two antisera were obtained using recombinant Imp proteins from two FD strains. Preliminary results confirmed the specificity and good ELISA performance in the detection of the target in grapevine, clematis and insect vectors tissues.",

keywords = "INHS, Leafhopper, Grapevine yellows, Serology, Phytoplasma, ELISA",

author = "Luisa Filippin and Valeria Trivellone and Luciana Galetto and Cristina Marzach{\'i} and Vito Elicio and Elisa Angelini",

note = "Funding Information: This work was partly supported by the TROPICSAFE H2020 Project (contract No. 727459) and by the Swiss National Science Foundation (P2NEP3_168526/3). The nt and aa Imp sequences of 16SrV group phytoplasmas and the detection systems by PCR and ELISA are patented (European patent no. 2918685, Italian patent no. 1429213). Publisher Copyright: {\textcopyright} 2019, Technology Society of Basic and Applied Sciences. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2019",

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doi = "10.5958/2249-4677.2019.00038.0",

language = "English (US)",

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AU - Galetto, Luciana

AU - Marzachí, Cristina

AU - Elicio, Vito

AU - Angelini, Elisa

N1 - Funding Information: This work was partly supported by the TROPICSAFE H2020 Project (contract No. 727459) and by the Swiss National Science Foundation (P2NEP3_168526/3). The nt and aa Imp sequences of 16SrV group phytoplasmas and the detection systems by PCR and ELISA are patented (European patent no. 2918685, Italian patent no. 1429213). Publisher Copyright: © 2019, Technology Society of Basic and Applied Sciences. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019/6

Y1 - 2019/6

N2 - At present serological assays for the detection of “flavescence dorée” (FD), a quarantine disease of grapevine, are not available. The aim of this study was to produce polyclonal antibodies targeting a surface abundant protein of FD and related phytoplasmas, Imp (immunodominant membrane protein), and to verify its use for the detection of FD phytoplasmas in grapevine, other host plant species, and insect vectors. Two antisera were obtained using recombinant Imp proteins from two FD strains. Preliminary results confirmed the specificity and good ELISA performance in the detection of the target in grapevine, clematis and insect vectors tissues.

AB - At present serological assays for the detection of “flavescence dorée” (FD), a quarantine disease of grapevine, are not available. The aim of this study was to produce polyclonal antibodies targeting a surface abundant protein of FD and related phytoplasmas, Imp (immunodominant membrane protein), and to verify its use for the detection of FD phytoplasmas in grapevine, other host plant species, and insect vectors. Two antisera were obtained using recombinant Imp proteins from two FD strains. Preliminary results confirmed the specificity and good ELISA performance in the detection of the target in grapevine, clematis and insect vectors tissues.

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Development of an anti-Imp serological assay for the detection of “flavescence dorée” phytoplasmas in grapevine, insect vectors and host plants

Abstract

Keywords

ASJC Scopus subject areas

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