Development of an anti-Imp serological assay for the detection of “flavescence dorée” phytoplasmas in grapevine, insect vectors and host plants

Luisa Filippin, Valeria Trivellone, Luciana Galetto, Cristina Marzachí, Vito Elicio, Elisa Angelini

Research output: Contribution to journalArticlepeer-review

Abstract

At present serological assays for the detection of “flavescence dorée” (FD), a quarantine disease of grapevine, are not available. The aim of this study was to produce polyclonal antibodies targeting a surface abundant protein of FD and related phytoplasmas, Imp (immunodominant membrane protein), and to verify its use for the detection of FD phytoplasmas in grapevine, other host plant species, and insect vectors. Two antisera were obtained using recombinant Imp proteins from two FD strains. Preliminary results confirmed the specificity and good ELISA performance in the detection of the target in grapevine, clematis and insect vectors tissues.
Original languageEnglish (US)
Pages (from-to)75-76
Number of pages2
JournalPhytopathogenic Mollicutes
Volume9
Issue number1
DOIs
StatePublished - Jun 2019

Keywords

  • INHS
  • Leafhopper
  • Grapevine yellows
  • Serology
  • Phytoplasma
  • ELISA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Ecology, Evolution, Behavior and Systematics
  • Cell Biology
  • Parasitology

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