Desenvolvimento de um ensaio de Reação de Polimerase em Cadeia para detecção do vírus da pseudo-raiva em amostras clínicas

Translated title of the contribution: Development of a polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples

Lester J. Pérez, Heidy Díaz de Arce

Research output: Contribution to journalArticlepeer-review

Abstract

Aujeszky's disease, also known as pseudorabies causes severe economic losses in swine industry and affects the pig husbandry all over the world. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. The development, optimization and evaluation of a polymerase chain reaction (PCR) assay are presented for the diagnosis of pseudorabies infection. This assay was based on the amplification of a highly conserved viral gD gene fragment. PCR products of the expected size were obtained from PRV strains. Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. The analytical sensitivity of the test was estimated to be 1.34 TCID50/ 50 uL. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided.

Translated title of the contributionDevelopment of a polymerase chain reaction assay for the detection of pseudorabies virus in clinical samples
Original languagePortuguese
Pages (from-to)433-438
Number of pages6
JournalBrazilian Journal of Microbiology
Volume40
Issue number3
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Aujeszky's disease
  • PCR assay
  • Pseudorabies infection

ASJC Scopus subject areas

  • Microbiology

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