Development of a mitochondrial 12S rDNA analysis for distinguishing sciuridae species with potential to transmit Ehrlichia and Borrelia species to feeding Amblyomma americanum (Acari: Ixodidae)

Lisa S. Goessling, Brian F. Allan, Rachel S. Mandelbaum, Robert E. Thach

Research output: Contribution to journalArticle

Abstract

Unique oligonucleotide probes were synthesized to distinguish among closely related vertebrate mitochondrial rDNA sequences present in residual bloodmeals in emergent Amblyomma americanum (L.) (Acari: Ixodidae) nymph life-stage ticks. Use of these probes enabled the identification of the Eastern gray squirrel as an important bloodmeal source in nymphs harboring Ehrlichia and Borrelia species. These results were confirmed by identifying these same bacterial genera in Eastern gray squirrel tissues.

Original languageEnglish (US)
Pages (from-to)772-776
Number of pages5
JournalJournal of medical entomology
Volume49
Issue number3
DOIs
StatePublished - May 2012

Keywords

  • Amblyomma
  • Borrelia
  • Ehrlichia
  • Sciuridae
  • tick

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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