Development of a mitochondrial 12S rDNA analysis for distinguishing sciuridae species with potential to transmit Ehrlichia and Borrelia species to feeding Amblyomma americanum (Acari: Ixodidae)

Lisa S. Goessling; Brian F. Allan; Rachel S. Mandelbaum; Robert E. Thach

doi:10.1603/ME11203

Development of a mitochondrial 12S rDNA analysis for distinguishing sciuridae species with potential to transmit Ehrlichia and Borrelia species to feeding Amblyomma americanum (Acari: Ixodidae)

Lisa S. Goessling, Brian F. Allan, Rachel S. Mandelbaum, Robert E. Thach

Research output: Contribution to journal › Article › peer-review

Abstract

Unique oligonucleotide probes were synthesized to distinguish among closely related vertebrate mitochondrial rDNA sequences present in residual bloodmeals in emergent Amblyomma americanum (L.) (Acari: Ixodidae) nymph life-stage ticks. Use of these probes enabled the identification of the Eastern gray squirrel as an important bloodmeal source in nymphs harboring Ehrlichia and Borrelia species. These results were confirmed by identifying these same bacterial genera in Eastern gray squirrel tissues.

Original language	English (US)
Pages (from-to)	772-776
Number of pages	5
Journal	Journal of medical entomology
Volume	49
Issue number	3
DOIs	https://doi.org/10.1603/ME11203
State	Published - May 2012

Keywords

Amblyomma
Borrelia
Ehrlichia
Sciuridae
tick

ASJC Scopus subject areas

Parasitology
General Veterinary
Insect Science
Infectious Diseases

Online availability

10.1603/ME11203

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Development of a mitochondrial 12S rDNA analysis for distinguishing sciuridae species with potential to transmit Ehrlichia and Borrelia species to feeding Amblyomma americanum (Acari: Ixodidae). / Goessling, Lisa S.; Allan, Brian F.; Mandelbaum, Rachel S. et al.
In: Journal of medical entomology, Vol. 49, No. 3, 05.2012, p. 772-776.

Research output: Contribution to journal › Article › peer-review

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AU - Thach, Robert E.

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Development of a mitochondrial 12S rDNA analysis for distinguishing sciuridae species with potential to transmit Ehrlichia and Borrelia species to feeding Amblyomma americanum (Acari: Ixodidae)

Abstract

Keywords

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