Development of a cost-effective glucose-corn steep medium for production of butanol by Clostridium beijerinckii

M. Parekh, J. Formanek, H. P. Blaschek

Research output: Contribution to journalArticlepeer-review

Abstract

Corn steep water (CSW) medium (1.6% solids plus 6% glucose) was evaluated for growth and butanol production by Clostridium beijerinckii NCIMB 8052 wild-type and hyper-amylolytic, hyper-butanol-producing mutant strain BA1O1. CSW alone was not a suitable substrate, whereas addition of glucose supported growth and butanol production by both strains. In a batch-scale fermentation using an optimized 6% glucose-1.6% solids CSW medium, C. beijerinckii NCIMB 8052 and strain BA101 produced 10.7 gL-1 and 14.5 gL-1 of butanol, respectively. The total solvents (acetone, butanol, and ethanol) produced by C. beijerinckii NCIMB 8052 and strain BA1O1 were 14 gL-1 and 20 gL-1, respectively. Initial fermentation in small-scale flasks containing 6% maltodextrin-1.6% solids concentration CSW medium resulted in 6 gL-1 and 12.6 gL-1 of butanol production by C. beijerinckii NCIMB 8052 and strain BA101, respectively. CSW can serve as an economic source of nitrogen, vitamins, amino acids, minerals, and other nutrients. Thus, it is feasible to use 6% glucose-1.6% solids CSW medium in place of semi-defined P2 medium.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalJournal of Industrial Microbiology and Biotechnology
Volume21
Issue number4-5
DOIs
StatePublished - 1998

Keywords

  • Butanol
  • Clostridium beijerinckii
  • Corn steep water
  • Solvent production

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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