Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

Hee Sun Han, Neal K. Devaraj, Jungmin Lee, Scott A. Hilderbrand, Ralph Weissleder, Moungi G. Bawendi

Research output: Contribution to journalArticlepeer-review

Abstract

We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

Original languageEnglish (US)
Pages (from-to)7838-7839
Number of pages2
JournalJournal of the American Chemical Society
Volume132
Issue number23
DOIs
StatePublished - Jun 16 2010
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry
  • Biochemistry
  • Colloid and Surface Chemistry

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