Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

Hee Sun Han; Neal K. Devaraj; Jungmin Lee; Scott A. Hilderbrand; Ralph Weissleder; Moungi G. Bawendi

doi:10.1021/ja101677r

Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

Hee Sun Han, Neal K. Devaraj, Jungmin Lee, Scott A. Hilderbrand, Ralph Weissleder, Moungi G. Bawendi

Research output: Contribution to journal › Article › peer-review

Abstract

We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

Original language	English (US)
Pages (from-to)	7838-7839
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	132
Issue number	23
DOIs	https://doi.org/10.1021/ja101677r
State	Published - Jun 16 2010
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Online availability

10.1021/ja101677r

Library availability

Discover UIUC Full Text

Cite this

@article{8e2615fb18184795aca4bbfe807ee37c,

title = "Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition",

abstract = "We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.",

author = "Han, {Hee Sun} and Devaraj, {Neal K.} and Jungmin Lee and Hilderbrand, {Scott A.} and Ralph Weissleder and Bawendi, {Moungi G.}",

year = "2010",

month = jun,

day = "16",

doi = "10.1021/ja101677r",

language = "English (US)",

volume = "132",

pages = "7838--7839",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "23",

}

TY - JOUR

T1 - Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

AU - Han, Hee Sun

AU - Devaraj, Neal K.

AU - Lee, Jungmin

AU - Hilderbrand, Scott A.

AU - Weissleder, Ralph

AU - Bawendi, Moungi G.

PY - 2010/6/16

Y1 - 2010/6/16

N2 - We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

AB - We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

UR - http://www.scopus.com/inward/record.url?scp=77953299417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953299417&partnerID=8YFLogxK

U2 - 10.1021/ja101677r

DO - 10.1021/ja101677r

M3 - Article

C2 - 20481508

AN - SCOPUS:77953299417

SN - 0002-7863

VL - 132

SP - 7838

EP - 7839

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 23

ER -

Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this