Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

Hee Sun Han; Neal K. Devaraj; Jungmin Lee; Scott A. Hilderbrand; Ralph Weissleder; Moungi G. Bawendi

doi:10.1021/ja101677r

Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

Hee Sun Han, Neal K. Devaraj, Jungmin Lee, Scott A. Hilderbrand, Ralph Weissleder, Moungi G. Bawendi

Research output: Contribution to journal › Article › peer-review

Abstract

We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

Original language	English (US)
Pages (from-to)	7838-7839
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	132
Issue number	23
DOIs	https://doi.org/10.1021/ja101677r
State	Published - Jun 16 2010
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja101677r

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AU - Lee, Jungmin

AU - Hilderbrand, Scott A.

AU - Weissleder, Ralph

AU - Bawendi, Moungi G.

PY - 2010/6/16

Y1 - 2010/6/16

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AB - We present a bioorthogonal and modular conjugation method for efficient coupling of organic dyes and biomolecules to quantum dots (QDs) using a norbornene-tetrazine cycloaddition. The use of noncoordinating functional groups combined with the rapid rate of the cycloaddition leads to highly efficient conjugation. We have applied this method to the in situ targeting of norbornene-coated QDs to live cancer cells labeled with tetrazine-modified proteins.

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Development of a bioorthogonal and highly efficient conjugation method for quantum dots using tetrazine-norbornene cycloaddition

Abstract

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