Development of a biomaterial model of the decidualized endometrium

Samantha G. Zambuto, Brendan A Harley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: The endometrium, the site of embryo implantation, is a unique, dynamic tissue that undergoes rapid vascular remodeling over the course of the menstrual cycle and pregnancy. Endometrial models are crucial for understanding reproductive disorders, including preeclampsia and endometriosis. They also provide a platform to examine mechanisms underlying vascular remodeling that takes place within the endometrium. While animal models can be used to study dynamic endometrial processes, physiological differences in placentation between animals and humans call into question the relevance of such models. Traditional 2D in vitro models allow researchers to study the endometrium using human cells; however, they do not capture cellular crosstalk in complex microenvironments. Tissue engineered 3D endometrial models would provide new avenues to recapitulate aspects of the in vivo environment while retaining the capability to study cell-cell and cell-matrix interactions in a highly tunable manner. Herein, we describe an adaptable 3D hydrogel model of the endometrium consisting of a coculture of human endometrial stromal cells (HESCs) and human umbilical vein endothelial cells (HUVECs) within a methacrylamide-functionalized gelatin (GelMA) hydrogel. We demonstrate the ability to form vessel structures within this model as well as study the kinetics of trophoblast invasion in 3D. The GelMA hydrogel provides a platform to integrate additional signals from the native tissue microenvironment of the endometrium, namely sex steroid hormones, to examine how the role played by endometrial stromal cell decidualization status (specialized decidual endometrial stromal cells play a critical role in embryo implantation and placentation) affects endothelial cell network formation.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

Fingerprint

Biocompatible Materials
Endometrium
Biomaterials
Hydrogel
Stromal Cells
Placentation
Hydrogels
Endothelial cells
Tissue
Physiological Phenomena
Animals
Human Umbilical Vein Endothelial Cells
Trophoblasts
Gonadal Steroid Hormones
Steroid hormones
Endometriosis
Gelatin
Menstrual Cycle
Coculture Techniques
Pre-Eclampsia

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Zambuto, S. G., & Harley, B. A. (2019). Development of a biomaterial model of the decidualized endometrium. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.

Development of a biomaterial model of the decidualized endometrium. / Zambuto, Samantha G.; Harley, Brendan A.

Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zambuto, SG & Harley, BA 2019, Development of a biomaterial model of the decidualized endometrium. in Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, vol. 40, Society for Biomaterials, 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence, Seattle, United States, 4/3/19.
Zambuto SG, Harley BA. Development of a biomaterial model of the decidualized endometrium. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials. 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
Zambuto, Samantha G. ; Harley, Brendan A. / Development of a biomaterial model of the decidualized endometrium. Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
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