Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

Luis G. Giménez-Lirola; Priscilla F. Gerber; Raymond R. Rowland; Patrick G. Halbur; Yao Wei Huang; Xiang Jin Meng; Tanja Opriessnig

doi:10.1016/j.rvsc.2014.02.014

Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

Luis G. Giménez-Lirola, Priscilla F. Gerber, Raymond R. Rowland, Patrick G. Halbur, Yao Wei Huang, Xiang Jin Meng, Tanja Opriessnig

Research output: Contribution to journal › Article › peer-review

Abstract

A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem.

Original language	English (US)
Pages (from-to)	543-550
Number of pages	8
Journal	Research in Veterinary Science
Volume	96
Issue number	3
DOIs	https://doi.org/10.1016/j.rvsc.2014.02.014
State	Published - Jun 2014
Externally published	Yes

Keywords

4-plex
Antibody detection
Fluorescent microbead-based immunoassay
Porcine reproductive and respiratory syndrome virus
PRRSV
Torque teno sus virus
TTSuV

ASJC Scopus subject areas

General Veterinary

Online availability

10.1016/j.rvsc.2014.02.014

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Giménez-Lirola, L. G., Gerber, P. F., Rowland, R. R., Halbur, P. G., Huang, Y. W., Meng, X. J., & Opriessnig, T. (2014). Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. Research in Veterinary Science, 96(3), 543-550. https://doi.org/10.1016/j.rvsc.2014.02.014

Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. / Giménez-Lirola, Luis G.; Gerber, Priscilla F.; Rowland, Raymond R. et al.
In: Research in Veterinary Science, Vol. 96, No. 3, 06.2014, p. 543-550.

Research output: Contribution to journal › Article › peer-review

Giménez-Lirola, LG, Gerber, PF, Rowland, RR, Halbur, PG, Huang, YW, Meng, XJ & Opriessnig, T 2014, 'Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2', Research in Veterinary Science, vol. 96, no. 3, pp. 543-550. https://doi.org/10.1016/j.rvsc.2014.02.014

Giménez-Lirola LG, Gerber PF, Rowland RR, Halbur PG, Huang YW, Meng XJ et al. Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. Research in Veterinary Science. 2014 Jun;96(3):543-550. doi: 10.1016/j.rvsc.2014.02.014

Giménez-Lirola, Luis G. ; Gerber, Priscilla F. ; Rowland, Raymond R. et al. / Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2. In: Research in Veterinary Science. 2014 ; Vol. 96, No. 3. pp. 543-550.

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abstract = "A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem.",

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AU - Halbur, Patrick G.

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Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

Abstract

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