Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2

Luis G. Giménez-Lirola, Priscilla F. Gerber, Raymond R. Rowland, Patrick G. Halbur, Yao Wei Huang, Xiang Jin Meng, Tanja Opriessnig

Research output: Contribution to journalArticlepeer-review

Abstract

A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem.

Original languageEnglish (US)
Pages (from-to)543-550
Number of pages8
JournalResearch in Veterinary Science
Volume96
Issue number3
DOIs
StatePublished - Jun 2014
Externally publishedYes

Keywords

  • 4-plex
  • Antibody detection
  • Fluorescent microbead-based immunoassay
  • Porcine reproductive and respiratory syndrome virus
  • PRRSV
  • Torque teno sus virus
  • TTSuV

ASJC Scopus subject areas

  • veterinary(all)

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