TY - JOUR
T1 - Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2
AU - Giménez-Lirola, Luis G.
AU - Gerber, Priscilla F.
AU - Rowland, Raymond R.
AU - Halbur, Patrick G.
AU - Huang, Yao Wei
AU - Meng, Xiang Jin
AU - Opriessnig, Tanja
N1 - Funding Information:
This study was funded by the Iowa Livestock Health Advisory Council .
PY - 2014/6
Y1 - 2014/6
N2 - A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem.
AB - A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem.
KW - 4-plex
KW - Antibody detection
KW - Fluorescent microbead-based immunoassay
KW - Porcine reproductive and respiratory syndrome virus
KW - PRRSV
KW - Torque teno sus virus
KW - TTSuV
UR - http://www.scopus.com/inward/record.url?scp=84899986281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899986281&partnerID=8YFLogxK
U2 - 10.1016/j.rvsc.2014.02.014
DO - 10.1016/j.rvsc.2014.02.014
M3 - Article
C2 - 24650623
AN - SCOPUS:84899986281
VL - 96
SP - 543
EP - 550
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 3
ER -