Development and Use of a Monoclonal Antibody Specific for the Candida albicans Cell-Surface Protein Hwp1

Soon Hwan Oh, Hélène Martin-Yken, David A. Coleman, Etienne Dague, Lois L. Hoyer

Research output: Contribution to journalArticlepeer-review

Abstract

The Candida albicans cell-surface protein Hwp1 functions in adhesion to the host and in biofilm formation. A peptide from the Gln-Pro-rich adhesive domain of Hwp1 was used to raise monoclonal antibody (MAb) 2-E8. MAb 2-E8 specificity for Hwp1 was demonstrated using a hwp1/hwp1 C. albicans isolate and strains that expressed at least one HWP1 allele. Immunofluorescence and atomic force microscopy experiments using MAb 2-E8 confirmed C. albicans germ-tube-specific detection of the Hwp1 protein. MAb 2-E8 also immunolabeled the tips of some Candida dubliniensis germ tubes grown under conditions that maximized HWP1 expression. The phylogeny of HWP1 and closely related genes suggested that the Gln-Pro-rich adhesive domain was unique to C. albicans and C. dubliniensis focusing the utility of MAb 2-E8 on these species. This new reagent can be used to address unanswered questions about Hwp1 and its interactions with other proteins in the context of C. albicans biology and pathogenesis.

Original languageEnglish (US)
Article number907453
JournalFrontiers in Cellular and Infection Microbiology
Volume12
DOIs
StatePublished - Jun 27 2022

Keywords

  • Candida albicans
  • Hwp1
  • adhesion
  • biofilm formation
  • cell-surface protein
  • monoclonal antibody

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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