Development and mapping of microsatellites from a microdissected bta 11-specific dna library

S. Ambady, S. M. Kappes, C. Park, R. Z. Ma, J. E. Beever, H. A. Lewin, T. P.L. Smith, C. W. Beattie, P. K. Basrur, F. De Abel Ponce León

Research output: Contribution to journalArticle

Abstract

A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.

Original languageEnglish (US)
Pages (from-to)152-155
Number of pages4
JournalAnimal genetics
Volume32
Issue number3
DOIs
StatePublished - Aug 29 2001

Keywords

  • Bovine chromosome 11
  • Chromosome microdissection
  • Linkage map
  • Microsatellite markers

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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    Ambady, S., Kappes, S. M., Park, C., Ma, R. Z., Beever, J. E., Lewin, H. A., Smith, T. P. L., Beattie, C. W., Basrur, P. K., & De Abel Ponce León, F. (2001). Development and mapping of microsatellites from a microdissected bta 11-specific dna library. Animal genetics, 32(3), 152-155. https://doi.org/10.1046/j.1365-2052.2001.00744.x