Development and mapping of microsatellites from a microdissected bta 11-specific dna library

S. Ambady; S. M. Kappes; C. Park; R. Z. Ma; J. E. Beever; H. A. Lewin; T. P.L. Smith; C. W. Beattie; P. K. Basrur; F. De Abel Ponce León

doi:10.1046/j.1365-2052.2001.00744.x

Development and mapping of microsatellites from a microdissected bta 11-specific dna library

S. Ambady, S. M. Kappes, C. Park, R. Z. Ma, J. E. Beever, H. A. Lewin, T. P.L. Smith, C. W. Beattie, P. K. Basrur, F. De Abel Ponce León

Animal Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)₁₂ oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.

Original language	English (US)
Pages (from-to)	152-155
Number of pages	4
Journal	Animal genetics
Volume	32
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-2052.2001.00744.x
State	Published - 2001

Keywords

Bovine chromosome 11
Chromosome microdissection
Linkage map
Microsatellite markers

ASJC Scopus subject areas

Animal Science and Zoology
Genetics

Online availability

10.1046/j.1365-2052.2001.00744.x

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abstract = "A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.",

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language = "English (US)",

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AU - Ambady, S.

AU - Kappes, S. M.

AU - Park, C.

AU - Ma, R. Z.

AU - Beever, J. E.

AU - Lewin, H. A.

AU - Smith, T. P.L.

AU - Beattie, C. W.

AU - Basrur, P. K.

AU - De Abel Ponce León, F.

PY - 2001

Y1 - 2001

N2 - A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.

AB - A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.

KW - Bovine chromosome 11

KW - Chromosome microdissection

KW - Linkage map

KW - Microsatellite markers

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Development and mapping of microsatellites from a microdissected bta 11-specific dna library

Abstract

Keywords

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