Development and GBS-genotyping of introgression lines (ILs) using two wild species of rice, O. meridionalis and O. rufipogon, in a common recurrent parent, O. sativa cv. Curinga

Juan D. Arbelaez, Laura T. Moreno, Namrata Singh, Chih Wei Tung, Lyza G. Maron, Yolima Ospina, César P. Martinez, Cécile Grenier, Mathias Lorieux, Susan McCouch

Research output: Contribution to journalArticle

Abstract

Two populations of interspecific introgression lines (ILs) in a common recurrent parent were developed for use in pre-breeding and QTL mapping. The ILs were derived from crosses between cv Curinga, a tropical japonica upland cultivar, and two different wild donors, Oryza meridionalis Ng. accession (W2112) and Oryza rufipogon Griff. accession (IRGC 105491). The lines were genotyped using genotyping-by-sequencing (GBS) and SSRs. The 32 Curinga/O. meridionalis ILs contain 76.73 % of the donor genome in individual introgressed segments, and each line has an average of 94.9 % recurrent parent genome. The 48 Curinga/O. rufipogon ILs collectively contain 97.6 % of the donor genome with an average of 89.9 % recurrent parent genome per line. To confirm that these populations were segregating for traits of interest, they were phenotyped for pericarp color in the greenhouse and for four agronomic traits—days to flowering, plant height, number of tillers, and number of panicles—in an upland field environment. Seeds from these IL libraries and the accompanying GBS datasets are publicly available and represent valuable genetic resources for exploring the genetics and breeding potential of rice wild relatives.

Original languageEnglish (US)
JournalMolecular Breeding
Volume35
Issue number2
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • Allele discovery
  • Chromosome segment substitution lines (CSSLs)
  • Crop wild relatives
  • Genotyping by sequencing (GBS)
  • Marker-assisted selection (MAS)
  • Oryza sativa
  • Rice

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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