Development and characterization of an ELISA assay in PDMS microfluidic channels

E. Eteshola; D. Leckband

doi:10.1016/S0925-4005(00)00640-7

Development and characterization of an ELISA assay in PDMS microfluidic channels

Research output: Contribution to journal › Article › peer-review

Abstract

This report describes the first preliminary evaluation of a heterogeneous sandwich enzyme-linked immunoassay in a PDMS microfluidic device. The PDMS devices were fabricated using replica molding against a patterned photoresist generated by photolithographic techniques. With this experimental setup, the microfluidic sensor chip was successfully used to quantify a model analyte (sheep IgM) with sensitivity down to 17 nM. The conventional blocking cocktail used in nearly all polystyrene microtiter plate-based ELISA assays failed to block the nonspecific adsorption of the analyte and secondary antibody. This report describes the successful use of surface modification chemistries and a modified blocking cocktail to reduce background due to nonspecific adsorption and to thereby increase the sensitivity of the assay. These protocols can be extended to the detection of a variety of analytes by immunoassay in PDMS microchannels.

Original language	English (US)
Pages (from-to)	129-133
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	72
Issue number	2
DOIs	https://doi.org/10.1016/S0925-4005(00)00640-7
State	Published - Jan 25 2001

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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