Development and characterization of an ELISA assay in PDMS microfluidic channels

E. Eteshola, D. Leckband

Research output: Contribution to journalArticle

Abstract

This report describes the first preliminary evaluation of a heterogeneous sandwich enzyme-linked immunoassay in a PDMS microfluidic device. The PDMS devices were fabricated using replica molding against a patterned photoresist generated by photolithographic techniques. With this experimental setup, the microfluidic sensor chip was successfully used to quantify a model analyte (sheep IgM) with sensitivity down to 17 nM. The conventional blocking cocktail used in nearly all polystyrene microtiter plate-based ELISA assays failed to block the nonspecific adsorption of the analyte and secondary antibody. This report describes the successful use of surface modification chemistries and a modified blocking cocktail to reduce background due to nonspecific adsorption and to thereby increase the sensitivity of the assay. These protocols can be extended to the detection of a variety of analytes by immunoassay in PDMS microchannels.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalSensors and Actuators, B: Chemical
Volume72
Issue number2
DOIs
StatePublished - Jan 25 2001

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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