Development and annotation of perennial Triticeae ESTs and SSR markers

B. Shaun Bushman, Steve R. Larson, Ivan W. Mott, Paul F. Cliften, Richard R.C. Wang, N. Jerry Chatterton, Alvaro G. Hernandez, Shahjahan Ali, Ryan W. Kim, Jyothi Thimmapuram, George Gong, Lei Liu, Mark A. Mikel

Research output: Contribution to journalArticlepeer-review


Triticeae contains hundreds of species of both annual and perennial types. Although substantial genomic tools are available for annual Triticeae cereals such as wheat and barley, the perennial Triticeae lack sufficient genomic resources for genetic mapping or diversity research. To increase the amount of sequence information available in the perennial Triticeae, three expressed sequence tag (EST) libraries were developed and annotated for Pseudoroegneria spicata, a mixture of both Elymus wawawaiensis and E. lanceolatus, and a Leymus cinereus x L. triticoides interspecific hybrid. The ESTs were combined into unigene sets of 8 780 unigenes for P. spicata, 11 281 unigenes for Leymus, and 7 212 unigenes for Elymus. Unigenes were annotated based on putative orthology to genes from rice, wheat, barley, other Poaceae, Arabidopsis, and the non-redundant database of the NCBI. Simple sequence repeat (SSR) markers were developed, tested for amplification and polymorphism, and aligned to the rice genome. Leymus EST markers homologous to rice chromosome 2 genes were syntenous on Leymus homeologous groups 6a and 6b (previously 1b), demonstrating promise for in silico comparative mapping. All ESTs and SSR markers are available on an EST information management and annotation database (http://titan.biotec.uiuc. edu/triticeae/).

Original languageEnglish (US)
Pages (from-to)779-788
Number of pages10
Issue number10
StatePublished - Oct 2008


  • Comparative genomics
  • Elymus
  • Expressed sequence tag
  • Leymus
  • Microsatellite
  • Pseudoroegneria spicata
  • Simple sequence repeat
  • Triticeae

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics


Dive into the research topics of 'Development and annotation of perennial Triticeae ESTs and SSR markers'. Together they form a unique fingerprint.

Cite this